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| rdf:type
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| Description
| - We applied the nuclear DNA Diffusion Assay, described as an accurate tool to estimate apoptotic and necrotic cells [N.P. Singh, A simple method for accurate estimation of apoptotic cells, Exp. Cell Res. 256 (2000) 328-337] to tobacco root and leaf cells. In this assay, isolated nuclei are embedded in an agarose microgel on a microscope slide and low molecular-weight DNA fragments diffuse into the microgel. Exposure of the roots to hydrogen peroxide significantly increased the average nuclear area of isolated nuclei. After 4 and 24h of recovery, all DNA damage was repaired. The data clearly demonstrate that the manifestation of diffused nuclei upon exposure to hydrogen peroxide is not the result of non-repairable apo-ptotic or necrotic DNA fragmentation, but represents repairable genotoxin-induced DNA da-mage. In contrast, treatment with the alkylating agent ethyl methanesulphonate (EMS) fol-lowed by 24 h of recovery produced a significant increase in the average nuclear area. The co...
- We applied the nuclear DNA Diffusion Assay, described as an accurate tool to estimate apoptotic and necrotic cells [N.P. Singh, A simple method for accurate estimation of apoptotic cells, Exp. Cell Res. 256 (2000) 328-337] to tobacco root and leaf cells. In this assay, isolated nuclei are embedded in an agarose microgel on a microscope slide and low molecular-weight DNA fragments diffuse into the microgel. Exposure of the roots to hydrogen peroxide significantly increased the average nuclear area of isolated nuclei. After 4 and 24h of recovery, all DNA damage was repaired. The data clearly demonstrate that the manifestation of diffused nuclei upon exposure to hydrogen peroxide is not the result of non-repairable apo-ptotic or necrotic DNA fragmentation, but represents repairable genotoxin-induced DNA da-mage. In contrast, treatment with the alkylating agent ethyl methanesulphonate (EMS) fol-lowed by 24 h of recovery produced a significant increase in the average nuclear area. The co... (en)
- Difuzní jaderní test je doporučen k rozlišení apoptotického a nekrotického poškození DNA podle struktury jader u savčích buněk. Tento test jsme aplikovali na jádra z listů a kořenů tabáku (Nicotiana tabacum var. xanthi). Po aplikaci peroxidu vodíku na jádra z kořenů se průkazně zvýšil průměr jader, způsobený poškozením DNA, po 24 h však bylo poškození DNA kompletně napraveno. To dokazuje, že poškození DNA není podmíněno apoptózou nebo apoptózou , která není reparovatelná, ale reparovatelnou genotoxicitou. Ani po aplikaci DNase-1 a hyperthermie nebylo možno rozlišit podle struktury mezi apoptotickou, nekrotickou a genotoxickou fragmentaci DNA. (cs)
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| Title
| - Evaluation of the nuclear DNA Diffusion Assay to detect apoptosis and necrosis
- Hodnocení jaderného DNA difúzního testu k detekci apoptózy a apoptózy (cs)
- Evaluation of the nuclear DNA Diffusion Assay to detect apoptosis and necrosis (en)
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| skos:prefLabel
| - Evaluation of the nuclear DNA Diffusion Assay to detect apoptosis and necrosis
- Hodnocení jaderného DNA difúzního testu k detekci apoptózy a apoptózy (cs)
- Evaluation of the nuclear DNA Diffusion Assay to detect apoptosis and necrosis (en)
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| skos:notation
| - RIV/61389030:_____/05:00024908!RIV06-GA0-61389030
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA521/05/0500), Z(AV0Z50380511)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/05:00024908
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - DNase-I; Ethyl methanesulphonate; Hydrogen peroxide (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Mukherjee, A.
- Gichner, Tomáš
- Plewa, M. J.
- Wagner, E. D.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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