| Attributes | Values |
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| rdf:type
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| Description
| - Cytokininy mají rozhodující úlohu v regulaci vývoje a diferenciace plastidů, ale jejich metabolismus v plastidech není dosud znám. Metabolické studie s intaktními chloroplasty nelze provádět z důvodu nestability izolovaných chloroplastů. Proto byly chloroplasty izolované z tabáku (Nicotiana tabacum L. cv. Petit Havana SR1) imobilizovány v alginátu s nízkou viskozitou. Jejich intaktnost byla sledována glyceraldehyd-3-fosfát-dehydrogenázovým testem, který ukázal, že volné chloroplasty jsou úplně dezintegrovány během 7 hodin, zatímco imobilizované chloroplasty jsou po 24 hodinách z 50 % intaktní. Imobilizace neměla žádný výrazný vliv na ultrastrukturu a prodloužila životnost chloroplastů. Profil metabolitů byl podobný ve volných i imobilizovaných chloroplastechm po 4h inkubaci s triciovaným zeatinem. Ale u imobilizovaných chloroplastů se výrazně snižuje (2 x) výtěžek produktů metabolismu, což je zřejmě důsledek ztížené difuse metabolitů gelem a sorpcí na polysacharidovou matrix. (cs)
- Cytokinins play a decisive role in regulation of plastid development and differentiation, but their metabolism in plastids is not known. Metabolic studies using intact chloroplasts are prevented by their instability once they are isolated from leaf cells. Chloroplasts of Nicotiana tabacum L. cv. Petit Havana SR1 were therefore immobilized into low-viscosity alginate. Their intactness was assessed by a glyceraldehyde-3-phosphate dehydrogenase assay which indicated that free chloroplasts totally disintegrated within 7 h, while more than 50% of immobilized chloroplasts remained intact after 24 h. The immobilization had no marked impact on ultrastructure and postponed final destruction. The metabolite profile was similar in free and immobilized chloroplasts after 4 h incubation with tritiated zeatin. Nevertheless, the yield of conversion products decreased twice in immobilized chloroplasts, which was probably the outcome of mass transfer limitations and/or the sorption to polysaccharide...
- Cytokinins play a decisive role in regulation of plastid development and differentiation, but their metabolism in plastids is not known. Metabolic studies using intact chloroplasts are prevented by their instability once they are isolated from leaf cells. Chloroplasts of Nicotiana tabacum L. cv. Petit Havana SR1 were therefore immobilized into low-viscosity alginate. Their intactness was assessed by a glyceraldehyde-3-phosphate dehydrogenase assay which indicated that free chloroplasts totally disintegrated within 7 h, while more than 50% of immobilized chloroplasts remained intact after 24 h. The immobilization had no marked impact on ultrastructure and postponed final destruction. The metabolite profile was similar in free and immobilized chloroplasts after 4 h incubation with tritiated zeatin. Nevertheless, the yield of conversion products decreased twice in immobilized chloroplasts, which was probably the outcome of mass transfer limitations and/or the sorption to polysaccharide... (en)
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| Title
| - Viability, ultrastructure and cytokinin metabolism of free and immobilized tobacco chloroplasts
- Životnost, ultrastruktura a metabolismus cytokininů ve volných a imobilizovaných chloroplastech tabáku (cs)
- Viability, ultrastructure and cytokinin metabolism of free and immobilized tobacco chloroplasts (en)
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| skos:prefLabel
| - Viability, ultrastructure and cytokinin metabolism of free and immobilized tobacco chloroplasts
- Životnost, ultrastruktura a metabolismus cytokininů ve volných a imobilizovaných chloroplastech tabáku (cs)
- Viability, ultrastructure and cytokinin metabolism of free and immobilized tobacco chloroplasts (en)
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| skos:notation
| - RIV/61389030:_____/04:00109754!RIV/2005/AV0/A56005/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA206/03/0369), P(LN00A081), P(OC 840.20), Z(AV0Z5038910)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/04:00109754
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - calcium alginate; chloroplast ultrastructure; cytokinin metabolism (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dobrev, Petre
- Macháčková, Ivana
- Vaňková, Radomíra
- Polanská, Lenka
- Vičánková, Anna
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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