About: Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression

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About: Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. Transformation of both genes into Arabidopsis and tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. Additional cloning of two gene fragments, and an in silico search in the public expressed sequence tag clone databases, revealed the presence of at least 13 more members of the CKX gene family in barley and wheat. The expression of three selected barley genes was analyzed by RT-PCR and found to be organ-specific with peak expression in mature kernels. One barley CKX (HvCKX2) was characterized in detail after heterologous expression in tobacco. Interestingly, this enzyme shows a pH optimum at 4.5 and a preference for cytokinin ribosides as substrates, which may indicate its vacuolar targeting. Different substrate specificities, and the pH profiles of cyto... The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. Transformation of both genes into Arabidopsis and tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. Additional cloning of two gene fragments, and an in silico search in the public expressed sequence tag clone databases, revealed the presence of at least 13 more members of the CKX gene family in barley and wheat. The expression of three selected barley genes was analyzed by RT-PCR and found to be organ-specific with peak expression in mature kernels. One barley CKX (HvCKX2) was characterized in detail after heterologous expression in tobacco. Interestingly, this enzyme shows a pH optimum at 4.5 and a preference for cytokinin ribosides as substrates, which may indicate its vacuolar targeting. Different substrate specificities, and the pH profiles of cyto... (en) Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression. (cs)
Title	Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression (en) Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression (cs)
skos:prefLabel	Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression (en) Cytokinin oxidase/dehydrogenase genes in barley and wheat. Cloning and heterologous expression (cs)
skos:notation	RIV/61389030:_____/04:00109747!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany	3990;4002
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/03/0979), Z(AV0Z5038910), Z(MSM 153100008)
http://linked.open...iv/cisloPeriodika	20
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Strnad, Miroslav Frébortová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	559208
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/04:00109747
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cereals; cloning; cytokinin oxidase/dehydrogenase (en)
http://linked.open.../riv/klicoveSlovo	cereals cytokinin oxidase/dehydrogenase cloning
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[F2D03EF3D8B7]
http://linked.open...i/riv/nazevZdroje	EUROPEAN JOURNAL OF BIOCHEMISTRY
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	271
http://linked.open...iv/tvurceVysledku	Schmülling, T. Strnad, Miroslav Werner, T. Yamada, M. Frébortová, Jitka Galuszka, P. Frébort, I.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100008
issn	0014-2956
number of pages	13 (xsd:int)

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