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| Description
| - The first bacterial artificial chromosome (BAC) library of the banana species Musa balbisiana žPisang Klutuk WulungŽ (PKW BAC library) was constructed and characterized. One improved and one novel protocol for nuclei isolation were employed to overcome problems caused by high levels of polyphenols and polysaccharides present in leaf tissues. The use of flow cytometry to purify cell nuclei eliminated contamination with secondary metabolites and plastid DNA. Furthermore, the usefulness of the inducible pCC1BAC vector to obtain a higher amount of BAC DNA was demonstrated. The PKW BAC library represents nine haploid genome equivalents of M. balbisiana and its mean insert size is 135 kb. It consists of two sublibraries, of which the first one (SN sublibrary with 24 960 clones) was prepared according to an improved standard nuclei isolation protocol, whereas the second (FN sublibrary with 11 904 clones) was obtained from flow-sorted nuclei. Screening with 12 RFLP probes, which were geneti...
- The first bacterial artificial chromosome (BAC) library of the banana species Musa balbisiana žPisang Klutuk WulungŽ (PKW BAC library) was constructed and characterized. One improved and one novel protocol for nuclei isolation were employed to overcome problems caused by high levels of polyphenols and polysaccharides present in leaf tissues. The use of flow cytometry to purify cell nuclei eliminated contamination with secondary metabolites and plastid DNA. Furthermore, the usefulness of the inducible pCC1BAC vector to obtain a higher amount of BAC DNA was demonstrated. The PKW BAC library represents nine haploid genome equivalents of M. balbisiana and its mean insert size is 135 kb. It consists of two sublibraries, of which the first one (SN sublibrary with 24 960 clones) was prepared according to an improved standard nuclei isolation protocol, whereas the second (FN sublibrary with 11 904 clones) was obtained from flow-sorted nuclei. Screening with 12 RFLP probes, which were geneti... (en)
- Byla zkonstruována a charakterizována první knihovna dlouhých inzertů klonovaných ve vektoru BAC bananovníku Musa balbisiana kultivaru „Pisang Klutuk Wulung“. Byly použity dva protokoly izolace jader: 1) standardní protokol, 2) protokol využívající pro izolaci jader průtokovou cytometrii. Pomocí druhého jmenovaného protokolu se podařilo překonat obtíže při izolaci jader, které jsou způsobené přítomností sekundárních metabolitů v pletivech listů banánovníku. Knihovna představuje ekvivalent devíti haploidních genomů M. balbisiana. Vybrané klony byly použity pro lokalizaci na mitotických chromozómech pomocí fluorescenční in situ hybridizace. Knihovna může tedy být využívána pro izolaci cytogenetických markerů. BAC knihovna představuje veřejně dostupný cíl. V současnosti je používána pro studium integrace a aktivace BSV viru v genomu banánovníku a pro studium struktury a evoluce genomu nanánovníku. (cs)
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| Title
| - Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome
- Konstrukce BAC knihovny pro studium struktury a evoluce genomu banánovníku (M. balbisiana) (cs)
- Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome (en)
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| skos:prefLabel
| - Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome
- Konstrukce BAC knihovny pro studium struktury a evoluce genomu banánovníku (M. balbisiana) (cs)
- Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome (en)
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| skos:notation
| - RIV/61389030:_____/04:00109158!RIV/2005/AV0/A56005/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(IAA6038201), P(IAA6038204), Z(AV0Z5038910)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/04:00109158
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - bacterial artificial chromosome library; banana; BAC-FISH (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Doležel, Jaroslav
- Šafář, Jan
- Šimková, Hana
- Vrána, Jan
- Piffanelli, P.
- Alkhimova, Olena
- Caruana, M. L.
- Lheureux, F.
- Noa-Carrazana, J. C.
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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