| Attributes | Values |
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| rdf:type
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| Description
| - Cytoskeletální bílkoviny vytvářejí dynamické polymery, které se uplatňují v mnoha procesech, v buněčném dělení, signální transdukci a v determinaci tvaru a polarity buňky. Distribuce a dynamika mikrotubulů (MTs) a aktinových mikrofilament (AFs) jsou mimo jiné determinovány lokalizací jejich nukleačních míst. Zatímco víme, že místo pro nukleaci rostlinných mitrotubulů je pod plasmatickou membránou a během interfáze na jaderném obalu, tak informace o nukleačních místech AFs jsou zcela nedostatečná. Tato práce využívá nízkou teplotu (0°C) k depolymeraci AFs a MTs u interfázních BY-2 buněk tabáku (Nicotiana tabacum L.). Rozsah depolymerace cytoskeletálních filament během chladového působení a subcelulární distribuce nukleačních míst během následného zotavení v 25°C bylo hodnoceno fluorescenční mikroskopií. Výsledky ukazují, že AFs rychle repolymerovaly z míst lokalizovaných v kortikální oblasti a na jaderném obalu, tedy podobně jako MTs. Na rozdíl od MTs, před kompletní rekostitucí A... (cs)
- Cytoskeletal proteins assemble into dynamic polymers that play many roles in nuclear and cell division, signal transduction, and determination of cell shape and polarity. The distribution and dynamics of microtubules (MTs) and actin filaments (AFs) are determined, among other factors, by the location of their nucleation sites. Whereas the sites of microtubule nucleation in plants are known to be located under the plasma membrane and on the nuclear envelope during interphase, there is a striking lack of information about nucleation sites of AFs. In the studies reported herein, low temperature (0degreesC) was used to de-polymerize AFs and MTs in tobacco BY-2 (Nicotiana tabacum L.) cells at interphase. The extent of de-polymerization of cytoskeletal filaments in interphase cells during cold treatment and the subcellular distribution of nucleation sites during subsequent recovery at 25degreesC were monitored by means of fluorescence microscopy. The results show that AFs re-polymerized r...
- Cytoskeletal proteins assemble into dynamic polymers that play many roles in nuclear and cell division, signal transduction, and determination of cell shape and polarity. The distribution and dynamics of microtubules (MTs) and actin filaments (AFs) are determined, among other factors, by the location of their nucleation sites. Whereas the sites of microtubule nucleation in plants are known to be located under the plasma membrane and on the nuclear envelope during interphase, there is a striking lack of information about nucleation sites of AFs. In the studies reported herein, low temperature (0degreesC) was used to de-polymerize AFs and MTs in tobacco BY-2 (Nicotiana tabacum L.) cells at interphase. The extent of de-polymerization of cytoskeletal filaments in interphase cells during cold treatment and the subcellular distribution of nucleation sites during subsequent recovery at 25degreesC were monitored by means of fluorescence microscopy. The results show that AFs re-polymerized r... (en)
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| Title
| - Sites of actin filament initiation and reorganization in cold-treated tobacco cells
- Místa iniciace a reorganizace aktinových filament v buňkách tabáku po působení chladového stresu (cs)
- Sites of actin filament initiation and reorganization in cold-treated tobacco cells (en)
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| skos:prefLabel
| - Sites of actin filament initiation and reorganization in cold-treated tobacco cells
- Místa iniciace a reorganizace aktinových filament v buňkách tabáku po působení chladového stresu (cs)
- Sites of actin filament initiation and reorganization in cold-treated tobacco cells (en)
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| skos:notation
| - RIV/61389030:_____/04:00109157!RIV/2005/AV0/A56005/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(IAA5038207), Z(AV0Z5038910), Z(MSM 113100003)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/04:00109157
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Nicotiana tabacum; actin; actin filaments (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - PLANT CELL AND ENVIRONMENT
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Petrášek, Jan
- Janotová, I.
- Zelenková, S.
- Čapková, Věra
- Pokorná, J.
- Opatrný, Z.
- Schwarzerová, K.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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