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  • V buněčné suspenzní kultuře tabáku BY-2 byly studovány změny v buněčné viabilitě, proliferaci, buněčné a jaderné morfologii včetně jadernéa DNK fragmentace indukovaných působením kadmia po dobu 7 dnů. Současně byly sledovány změny v obsazích volných a konjugovaných forem polyaminů po dobu 3 dnů. Aplikace kadmia snížila buněčnou viabilitu jiz po 24 hodinách na 60% ve srovnání s kontrolou. Při delším působení dochází k rozpadu cytoplasmatických provazců, k tvorbě stresových granulí a mění se velikost a tvar jádra. Vystavení buněk tabáku BY-2 účinkům kadmia po dobu 3 dnů vyvolalo výrazný nárůst hladiny polyaminů, především putrescinu. (cs)
  • Changes in cell viability, proliferation, cell and nuclear morphology including nuclear and DNA fragmentation induced by 0.05 and 1 mM CdSO4 (Cd2+) in tobacco BY-2 cell line (Nicotiana tabacum L.) were studied in the course of 7 days. Simultaneously changes in endogenous contents of both free and conjugated forms of polyamines (PAs) were investigated for 3 days. The application of 0.05 mM Cd2+ evoked decline of cell viability to approximately 60% during the first 24 h of treatment. Later on degradation of cytoplasmic strands, formation of the stress granules and vesicles. modifications in size and shape of the nuclei, including their fragmentation. were observed in the surviving cells. Their proliferation was blocked and cells elongated. Beginning the first day of treatment TUNEL-positive nuclei were detected in cells cultivated in medium containing 0.05 mM Cd2+. Treatment with highly toxic 1 mM Cd2+ induced fast decrease of cell viability (no viable cells remained after 6-h treatme...
  • Changes in cell viability, proliferation, cell and nuclear morphology including nuclear and DNA fragmentation induced by 0.05 and 1 mM CdSO4 (Cd2+) in tobacco BY-2 cell line (Nicotiana tabacum L.) were studied in the course of 7 days. Simultaneously changes in endogenous contents of both free and conjugated forms of polyamines (PAs) were investigated for 3 days. The application of 0.05 mM Cd2+ evoked decline of cell viability to approximately 60% during the first 24 h of treatment. Later on degradation of cytoplasmic strands, formation of the stress granules and vesicles. modifications in size and shape of the nuclei, including their fragmentation. were observed in the surviving cells. Their proliferation was blocked and cells elongated. Beginning the first day of treatment TUNEL-positive nuclei were detected in cells cultivated in medium containing 0.05 mM Cd2+. Treatment with highly toxic 1 mM Cd2+ induced fast decrease of cell viability (no viable cells remained after 6-h treatme... (en)
Title
  • Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells
  • Cytologické změny a změny v obsazích polyaminů v buňkách tabáku BY-2 indukovaných kadmiem (cs)
  • Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells (en)
skos:prefLabel
  • Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells
  • Cytologické změny a změny v obsazích polyaminů v buňkách tabáku BY-2 indukovaných kadmiem (cs)
  • Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells (en)
skos:notation
  • RIV/61389030:_____/04:00109155!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany
  • 149;156
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LN00A081), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 559210
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/04:00109155
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • BY-2 cells; cadmium; DAO (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • FR - Francouzská republika
http://linked.open...ontrolniKodProRIV
  • [7134B919F875]
http://linked.open...i/riv/nazevZdroje
  • PLANT PHYSIOLOGY AND BIOCHEMISTRY
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 42
http://linked.open...iv/tvurceVysledku
  • Cvikrová, Milena
  • Macháčková, Ivana
  • Zelenková, S.
  • Opatrný, Z.
  • Gemperlová, Lenka
  • Eder, Josef
  • Kuthanová, A.
http://linked.open...n/vavai/riv/zamer
issn
  • 0981-9428
number of pages
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