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| Description
| - Nuclear genomes of some Triticeae species are characterized by large size and prevalence of repetitive DNA sequences. These features hamper their physical mapping and gene cloning. Purification of individual chromosomes by flow cytometry can simplify these tasks by providing small and defined genome fractions. This lecture reviews the development of the methodology and its potential for genome mapping in barley, rye and wheat Due to small differences in relative DNA content, only one chromosome type can be discriminated and sorted in each of the three species. Cytogenetic stocks facilitate separation of other parts of the genomes as individual chromosomes, translocation chromosomes and chromosome arms. Chromosome analysis by flow cytometry permits quantitative detection of structural and numerical chromosome changes. Chromosomes sorted onto microscopic slides have been used for discovery of rare structural changes and for high-resolution cytogenetic mapping using FISH. The use of so...
- Nuclear genomes of some Triticeae species are characterized by large size and prevalence of repetitive DNA sequences. These features hamper their physical mapping and gene cloning. Purification of individual chromosomes by flow cytometry can simplify these tasks by providing small and defined genome fractions. This lecture reviews the development of the methodology and its potential for genome mapping in barley, rye and wheat Due to small differences in relative DNA content, only one chromosome type can be discriminated and sorted in each of the three species. Cytogenetic stocks facilitate separation of other parts of the genomes as individual chromosomes, translocation chromosomes and chromosome arms. Chromosome analysis by flow cytometry permits quantitative detection of structural and numerical chromosome changes. Chromosomes sorted onto microscopic slides have been used for discovery of rare structural changes and for high-resolution cytogenetic mapping using FISH. The use of so... (en)
- Čeleď Triticeae je charakteristická velkým obsahem DNA repetitivní povahy. Tento fakt komplikuje jejich fyzické mapování a klonování genů. Izolace jednotlivých chromozomů průtokovou cytometrií umožňuje zjednodušit a zrychlit studium těchto genomů. Tříděných chromozomů může být využito pro HAPPY mapování, FISH techniky, mapování EST sekvencí, konstrukci chromozomově specifických BAC knihoven a řadě dalších. (cs)
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| Title
| - Dissecting large genomes of Triticeae by chromosome sorting
- Dissecting large genomes of Triticeae by chromosome sorting (en)
- Rozdělení velkých genomů čeledi Triticeae tříděných chromozomů (cs)
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| skos:prefLabel
| - Dissecting large genomes of Triticeae by chromosome sorting
- Dissecting large genomes of Triticeae by chromosome sorting (en)
- Rozdělení velkých genomů čeledi Triticeae tříděných chromozomů (cs)
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| skos:notation
| - RIV/61389030:_____/04:00107475!RIV/2005/AV0/A56005/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA521/04/0607), P(GA522/03/0354), Z(AV0Z5038910)
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/04:00107475
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Triticeae sp.; flow cytogenetics; FISH (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/nazevZdroje
| - Proceedings of the 9th International Barley Genetics Symposium
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Doležel, Jaroslav
- Kubaláková, Marie
- Suchánková, Pavla
- Šafář, Jan
- Číhalíková, Jarmila
- Šimková, Hana
- Janda, Jaroslav
- Kovářová, Pavlína
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Agricultural Research Institute, Kroměříž
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