| Attributes | Values |
|---|
| rdf:type
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| Description
| - Analýza genomu pšenice je komplikována jeho značnou velikostí (1C = 17 000 Mbp) a přítomností tří homeologních genomů (A, B a D) Jednou z možností jak analýzu genomu pšenice zjednodušit je konstrukce subgenomových DNA knihoven dlouhých inzertů. Pro konstrukci knihovny specificke pro chromozómy 1D, 4D a 6D bylo pomocí průtokové cytometrie purifikováno 10 milionů těchto chromozómů. Knihovna se skládá z 87 168 klonů s průměrnou velikostí inzertů 85 kbp a pokrývá chromozómy 1D, 4D a 6D 3,4x. Pravděpodobnost nalezení jakékoliv sekvence těchto tří chromozómů v dané knihovně je 96,5%. Tato knihovna je již druhou knihovnou v pořadí připravenou z tříděných rostlinných chromozómů a potvrzuje tedy skutečnost, že je možné zjednodušit analýzu komplexních genomů jejich rozdělením na části přípravou chromozomově popř. subgenomově specifických knihoven. (cs)
- The analysis of the hexaploid wheat genome (Triticum aestium L., 2n = 6x = 42) is hampered by its large size (16,974 Mb / 1C) and presence of three homoeologous genomes (A, B and D). One of the possible strategies is a targeted approach based on subgenomic libraries of large DNA inserts. In this work, we purified by flow cytometry a total of 107 of three wheat D-genome chromosomes: 1D, 4D and 6D. The library (designated as TA-subD) consists of 87,168 clones with an average insert size of 85 kb. Among these clones, 53 % had inserts larger than 100 kb, only 29% of inserts being shorter than 75 kb. The coverage was estimated to be 3.4x, giving a 96.5% probability of identifying a clone corresponding to any sequence on the three chromosomes. Specificity for chromosomes 1D, 4D and 6D was confirmed after screening the library pools with single-locus microsatellite markers. The screening indicated that the library was not biased and gave an estimated coverage of 6-fold. This is the second ...
- The analysis of the hexaploid wheat genome (Triticum aestium L., 2n = 6x = 42) is hampered by its large size (16,974 Mb / 1C) and presence of three homoeologous genomes (A, B and D). One of the possible strategies is a targeted approach based on subgenomic libraries of large DNA inserts. In this work, we purified by flow cytometry a total of 107 of three wheat D-genome chromosomes: 1D, 4D and 6D. The library (designated as TA-subD) consists of 87,168 clones with an average insert size of 85 kb. Among these clones, 53 % had inserts larger than 100 kb, only 29% of inserts being shorter than 75 kb. The coverage was estimated to be 3.4x, giving a 96.5% probability of identifying a clone corresponding to any sequence on the three chromosomes. Specificity for chromosomes 1D, 4D and 6D was confirmed after screening the library pools with single-locus microsatellite markers. The screening indicated that the library was not biased and gave an estimated coverage of 6-fold. This is the second ... (en)
|
| Title
| - Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat
- Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat (en)
- Konstrukce subgenomové BAC knihovny specifické pro chromozómy 1D, 4D a 6D hexaploidní pšenice (cs)
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| skos:prefLabel
| - Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat
- Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat (en)
- Konstrukce subgenomové BAC knihovny specifické pro chromozómy 1D, 4D a 6D hexaploidní pšenice (cs)
|
| skos:notation
| - RIV/61389030:_____/04:00107470!RIV/2005/AV0/A56005/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA521/04/0607), P(GA522/03/0354), Z(AV0Z5038910)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/04:00107470
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - wheat; subgenomic BAC library; flow cytometry (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Theoretical and Applied Genetics
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Doležel, Jaroslav
- Kubaláková, Marie
- Valárik, Miroslav
- Šafář, Jan
- Číhalíková, Jarmila
- Šimková, Hana
- Bernard, M.
- Sourdille, P.
- Janda, Jaroslav
- Chalhoub, B.
- Caboche, M.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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