Attributes | Values |
---|
rdf:type
| |
Description
| - The application of flow cytometry and sorting to plant chromosomes did not begin until the mid-1980s, having been delayed by difficulties in preparation of suspensions of intact chromosomes and discrimination of individual chromosome types.These problems have been overcome during the last ten years. So far, chromosome analysis and sorting has been reported in 17 species, including major legume and cereal crops. While chromosome classification by flow cytometry may be used for quantitative detection of structural and numerical chromosome changes, chromosomes purified by flow sorting were found to be invaluable in a broad range of applications. These included physical mapping using PCR, high-resolution cytogenetic mapping using FISH and PRINS, production of recombinant DNA libraries, targeted isolation of markers, and protein analysis. A great potential is foreseen for the use of sorted chromosomes for construction of chromosome and chromosome-arm-specific BAC libraries, targeted isol...
- The application of flow cytometry and sorting to plant chromosomes did not begin until the mid-1980s, having been delayed by difficulties in preparation of suspensions of intact chromosomes and discrimination of individual chromosome types.These problems have been overcome during the last ten years. So far, chromosome analysis and sorting has been reported in 17 species, including major legume and cereal crops. While chromosome classification by flow cytometry may be used for quantitative detection of structural and numerical chromosome changes, chromosomes purified by flow sorting were found to be invaluable in a broad range of applications. These included physical mapping using PCR, high-resolution cytogenetic mapping using FISH and PRINS, production of recombinant DNA libraries, targeted isolation of markers, and protein analysis. A great potential is foreseen for the use of sorted chromosomes for construction of chromosome and chromosome-arm-specific BAC libraries, targeted isol... (en)
- Aplikace průtokové cytometrie při izolaci rostlinných chromozomů se rozvíjí v posledních 10 letech. Byla aplikována u 17 rostlinných druhů, včetně významných hospodářských plodin. Tříděných chromozomů je využíváno v celé řadě molekulárně biologických analýzách. Mají výrazný potenciál pro konstrukci chromozomově specifických knihoven klonovaných ve vektoru BAC, pro fyzické mapování a genomiku rostlin obecně. (cs)
|
Title
| - Flow cytogenetics and plant genome mapping
- Průtoková cytogenetika a mapování geonomů rostlin (cs)
- Flow cytogenetics and plant genome mapping (en)
|
skos:prefLabel
| - Flow cytogenetics and plant genome mapping
- Průtoková cytogenetika a mapování geonomů rostlin (cs)
- Flow cytogenetics and plant genome mapping (en)
|
skos:notation
| - RIV/61389030:_____/04:00104357!RIV/2005/AV0/A56005/N
|
http://linked.open.../vavai/riv/strany
| |
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GA521/03/0595), P(GA522/03/0354), P(ME 527), P(ME 528), P(QC1336), Z(AV0Z5038910)
|
http://linked.open...iv/cisloPeriodika
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/04:00104357
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - Chromosome-specific DNA libraries; chromosome sorting; flow cytometry (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| |
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Doležel, Jaroslav
- Kubaláková, Marie
- Macas, Jiří
|
http://linked.open...n/vavai/riv/zamer
| |
issn
| |
number of pages
| |