About: Flow cytogenetics and plant genome mapping

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The application of flow cytometry and sorting to plant chromosomes did not begin until the mid-1980s, having been delayed by difficulties in preparation of suspensions of intact chromosomes and discrimination of individual chromosome types.These problems have been overcome during the last ten years. So far, chromosome analysis and sorting has been reported in 17 species, including major legume and cereal crops. While chromosome classification by flow cytometry may be used for quantitative detection of structural and numerical chromosome changes, chromosomes purified by flow sorting were found to be invaluable in a broad range of applications. These included physical mapping using PCR, high-resolution cytogenetic mapping using FISH and PRINS, production of recombinant DNA libraries, targeted isolation of markers, and protein analysis. A great potential is foreseen for the use of sorted chromosomes for construction of chromosome and chromosome-arm-specific BAC libraries, targeted isol... The application of flow cytometry and sorting to plant chromosomes did not begin until the mid-1980s, having been delayed by difficulties in preparation of suspensions of intact chromosomes and discrimination of individual chromosome types.These problems have been overcome during the last ten years. So far, chromosome analysis and sorting has been reported in 17 species, including major legume and cereal crops. While chromosome classification by flow cytometry may be used for quantitative detection of structural and numerical chromosome changes, chromosomes purified by flow sorting were found to be invaluable in a broad range of applications. These included physical mapping using PCR, high-resolution cytogenetic mapping using FISH and PRINS, production of recombinant DNA libraries, targeted isolation of markers, and protein analysis. A great potential is foreseen for the use of sorted chromosomes for construction of chromosome and chromosome-arm-specific BAC libraries, targeted isol... (en) Aplikace průtokové cytometrie při izolaci rostlinných chromozomů se rozvíjí v posledních 10 letech. Byla aplikována u 17 rostlinných druhů, včetně významných hospodářských plodin. Tříděných chromozomů je využíváno v celé řadě molekulárně biologických analýzách. Mají výrazný potenciál pro konstrukci chromozomově specifických knihoven klonovaných ve vektoru BAC, pro fyzické mapování a genomiku rostlin obecně. (cs)
Title	Flow cytogenetics and plant genome mapping Průtoková cytogenetika a mapování geonomů rostlin (cs) Flow cytogenetics and plant genome mapping (en)
skos:prefLabel	Flow cytogenetics and plant genome mapping Průtoková cytogenetika a mapování geonomů rostlin (cs) Flow cytogenetics and plant genome mapping (en)
skos:notation	RIV/61389030:_____/04:00104357!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany	77;91
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/03/0595), P(GA522/03/0354), P(ME 527), P(ME 528), P(QC1336), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Bartoš, Jan Doležel, Jaroslav Kubaláková, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	564586
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/04:00104357
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chromosome-specific DNA libraries; chromosome sorting; flow cytometry (en)
http://linked.open.../riv/klicoveSlovo	flow cytometry Chromosome-specific DNA libraries chromosome sorting
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[BDBC30762FFE]
http://linked.open...i/riv/nazevZdroje	Chromosome Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20528 Targeted isolation of molecular markers and comparative genome mapping in chickpea, field bean and garden pea Cytogenetic study of nuclear chromatin organization in plants Development and employment of new approaches for monitoring of genetic diversity and large-scale screening of genetic resources http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20527
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Bartoš, Jan Doležel, Jaroslav Kubaláková, Marie Macas, Jiří
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0967-3849
number of pages	15 (xsd:int)

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