About: Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlings     Goto   Sponge   NotDistinct   Permalink

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  • The role of cytokinin N-glucosylation and degradation by cytokinin oxidase/dehydrogenase (CKX, EC 1.5.99.12) in response to application of exogenous auxins (2,4-dichlorophenoxyacetic acid [2,4-D] and ?-naphthaleneacetic acid [NAA]) and cytokinins (N6-benzyladenine [BA] and trans-zeatin [Z]) was investigated in de-rooted seedlings of Raphanus sativus L. cv. Rampouch. Both auxins applied for 24 h at 1 and 10 ?M concentration increased N-glucosylation of exogenously applied [3H]dihydrozeatin (DHZ) by up to 20%. The level of endogenous 7N-glucosides (of Z, isopentenyladenine [iP] and DHZ) was increased by 2,4-D and NAA at 10 ?M concentration by 28 and 23%, respectively, the level of Z being decreased by 90 and 59%, respectively. 2,4-D and NAA suppressed CKX activity ca. by half. Exogenous cytokinins Z and BA applied at 1 and 10 ?M concentration stimulated 7N-glucosylation of [3H]DHZ (by up to 40%). BA both at 1 and 10 ?M, increased the level of endogenous Z by up to 35% and that of 7N-g...
  • The role of cytokinin N-glucosylation and degradation by cytokinin oxidase/dehydrogenase (CKX, EC 1.5.99.12) in response to application of exogenous auxins (2,4-dichlorophenoxyacetic acid [2,4-D] and ?-naphthaleneacetic acid [NAA]) and cytokinins (N6-benzyladenine [BA] and trans-zeatin [Z]) was investigated in de-rooted seedlings of Raphanus sativus L. cv. Rampouch. Both auxins applied for 24 h at 1 and 10 ?M concentration increased N-glucosylation of exogenously applied [3H]dihydrozeatin (DHZ) by up to 20%. The level of endogenous 7N-glucosides (of Z, isopentenyladenine [iP] and DHZ) was increased by 2,4-D and NAA at 10 ?M concentration by 28 and 23%, respectively, the level of Z being decreased by 90 and 59%, respectively. 2,4-D and NAA suppressed CKX activity ca. by half. Exogenous cytokinins Z and BA applied at 1 and 10 ?M concentration stimulated 7N-glucosylation of [3H]DHZ (by up to 40%). BA both at 1 and 10 ?M, increased the level of endogenous Z by up to 35% and that of 7N-g... (en)
  • Vliv aplikace exogenních auxinů (2,4-dicholorofenoxyoctové kyseliny [2,4-D] and ?-naftyloctové kyseliny [NAA]) a cytokininů (N6-benzyladeninu [BA] a trans-zeatinu [Z]) na N-glukosylaci cytokininů a jejich degradaci cytokinin oxidázou/dehydrogenázou (CKX, EC 1.5.99.12) byl sledován v nadzemních částech klíčních rostlin ředkvičky Raphanus sativus cv. Rampouch. Oba auxiny (v koncentraci 1 and 10 ?M) vedly po 24 hodinové inkubaci ke zvýšení N-glukosylace exogenního [3H]dihydrozeatinu (DHZ) až o 20%. Hladina endogenních 7N-glukosidů (Z, isopentenyladeninu [iP] a DHZ) byla zvýšena po aplikaci 10 ?M 2,4-D a 10 ?M NAA o 28%, respektive 23%. Přičemž hladina endogenního Z byla snížena o 90%, respektive 59%. 2,4-D a NAA snížily aktivitu CKX zhruba na polovinu. Exogenní cytokininy Z a BA aplikované v koncentraci 1 a 10 ?M stimulovaly 7N-glukosylaci [3H]DHZ (až o 40%). BA (1 ?M a 10 ?M) vedl ke zvýšení hladiny endogenního Z až o 35% a 7N-glukosidů až o 27%. Aplikace BA silně stimulovala aktivi... (cs)
Title
  • Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlings
  • Vliv exogenních cytokininů, auxinů a adeninu na N-glukosylaci cytokininů a aktivitu cytokinin oxidázy/dehydrogenázy v klíčních rostlinách ředkvičky (cs)
  • Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlings (en)
skos:prefLabel
  • Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlings
  • Vliv exogenních cytokininů, auxinů a adeninu na N-glukosylaci cytokininů a aktivitu cytokinin oxidázy/dehydrogenázy v klíčních rostlinách ředkvičky (cs)
  • Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlings (en)
skos:notation
  • RIV/61389030:_____/04:00104354!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany
  • 15;23
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/99/1130), P(IAA6038002), P(LN00A081), P(ME 505), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 561884
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/04:00104354
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Auxin; Cytokinin; Cytokinin oxidase/dehydrogenase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [E1CB6EB32141]
http://linked.open...i/riv/nazevZdroje
  • Plant Growth Regulation
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 44
http://linked.open...iv/tvurceVysledku
  • Dobrev, Petre
  • Malbeck, Jiří
  • Motyka, Václav
  • Vaňková, Radomíra
  • Gaudinová, Alena
  • Blagoeva, Elitsa
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-6903
number of pages
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