About: Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy.

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About: Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Using two-dimensional fluorescence spectroscopy, the growth of tobacco cells immobilized in alginate and pectate was monitored during the cultivation of the beads via the fluorescence of proteins (especially of tryptophan). Cell viability was followed by determination of their esterase activity using fluorescein diacetate as a substrate. This technique enables to measure the fluorescence of two fluorophores simultaneously. The effect of several stresses, (namely high and low temperature and the presence of organic solvent dimethylsulfoxide) was followed. Suitability of this technique to monitor the metabolic activities of cells within the beads was demonstrated. Using two-dimensional fluorescence spectroscopy, the growth of tobacco cells immobilized in alginate and pectate was monitored during the cultivation of the beads via the fluorescence of proteins (especially of tryptophan). Cell viability was followed by determination of their esterase activity using fluorescein diacetate as a substrate. This technique enables to measure the fluorescence of two fluorophores simultaneously. The effect of several stresses, (namely high and low temperature and the presence of organic solvent dimethylsulfoxide) was followed. Suitability of this technique to monitor the metabolic activities of cells within the beads was demonstrated. (en)
Title	Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy. Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy. (en)
skos:prefLabel	Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy. Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy. (en)
skos:notation	RIV/61389030:_____/03:56033145!RIV/2004/AV0/A56004/N
http://linked.open.../vavai/riv/strany	1;4
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(OC 840.20), Z(AV0Z4055905), Z(AV0Z4072921), Z(AV0Z5038910)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Vaňková, Radomíra Gaudinová, Alena
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	616356
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033145
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence spectroscopy; immobilization; dimethyl sulfoxide (en)
http://linked.open.../riv/klicoveSlovo	fluorescence spectroscopy immobilization dimethyl sulfoxide
http://linked.open...ontrolniKodProRIV	[F2C9E79B4344]
http://linked.open...v/mistoKonaniAkce	Illkirch [FR]
http://linked.open...i/riv/mistoVydani	France
http://linked.open...i/riv/nazevZdroje	Proceedings.
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20840.20
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Kuncová, Gabriela Podrazký, Ondřej Vaněk, Tomáš Vaňková, Radomíra Gaudinová, Alena
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2003-03-25 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function Theoretical fundamentals of chemical processes: equilibrium and dynamic behavior of multiphase reacting systems Chemical principles of selected biological phenomena in medicine and ecology
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Bioencapsulation Research Group

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