About: New cytokinin metabolites in IPT transgenic Arabidopsis thaliana plants.

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About: New cytokinin metabolites in IPT transgenic Arabidopsis thaliana plants. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two novel cytokinin metabolites were identified in transgenic Arabidopsis thaliana (L.) Heynh. plants containing the bacterial IPT gene under the transcriptional control of a heat-regulated promoter. After cyclic heat-shock treatment, the endogenous cytokinin concentrations were elevated up to 100-fold compared to the wild-type plants. More then 20 different cytokinin metabolites were found, with zeatin-type cytokinins being the most abundant. The metabolic inactivation of these compounds occurred predominantly through N-glucosylation. No significant accumulation of isopentenyladenine-type cytokinins, and only a small increase in dihydrozeatin metabolites, was observed. Subsequent studies of the abundant, unidentified conjugates revealed the presence of zeatin and dihydrozeatin diglucoside conjugates. Structural analysis, utilizing electrospray-liquid tandem mass spectrometry, identified these as a zeatin-O-glucoside-9-glucoside and dihydrozeatin-O-glucoside-9-glucoside, respectively. A third unknown Two novel cytokinin metabolites were identified in transgenic Arabidopsis thaliana (L.) Heynh. plants containing the bacterial IPT gene under the transcriptional control of a heat-regulated promoter. After cyclic heat-shock treatment, the endogenous cytokinin concentrations were elevated up to 100-fold compared to the wild-type plants. More then 20 different cytokinin metabolites were found, with zeatin-type cytokinins being the most abundant. The metabolic inactivation of these compounds occurred predominantly through N-glucosylation. No significant accumulation of isopentenyladenine-type cytokinins, and only a small increase in dihydrozeatin metabolites, was observed. Subsequent studies of the abundant, unidentified conjugates revealed the presence of zeatin and dihydrozeatin diglucoside conjugates. Structural analysis, utilizing electrospray-liquid tandem mass spectrometry, identified these as a zeatin-O-glucoside-9-glucoside and dihydrozeatin-O-glucoside-9-glucoside, respectively. A third unknown (en)
Title	New cytokinin metabolites in IPT transgenic Arabidopsis thaliana plants. New cytokinin metabolites in IPT transgenic Arabidopsis thaliana plants. (en)
skos:prefLabel	New cytokinin metabolites in IPT transgenic Arabidopsis thaliana plants. New cytokinin metabolites in IPT transgenic Arabidopsis thaliana plants. (en)
skos:notation	RIV/61389030:_____/03:56033121!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany	127;137
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/02/0475), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Werner, Tomáš Hanuš, Jan Holub, Petr Strnad, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	617822
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033121
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	transgenic Arabidopsis thaliana; cytokinin; zeatin-O-glucoside (en)
http://linked.open.../riv/klicoveSlovo	cytokinin zeatin-O-glucoside transgenic Arabidopsis thaliana
http://linked.open...odStatuVydavatele	DK - Dánské království
http://linked.open...ontrolniKodProRIV	[F91B93AC9F43]
http://linked.open...i/riv/nazevZdroje	Physiologia Plantarum
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Purine inhibitors of cyclin-dependent kinases as anticancer and antimitotic compounds
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	118
http://linked.open...iv/tvurceVysledku	Hanuš, Jan Holub, Petr Schmülling, T. Strnad, Miroslav Werner, Tomáš Onckelen, H. V.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0031-9317
number of pages	11 (xsd:int)

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