About: Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.     Goto   Sponge   NotDistinct   Permalink

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Description
  • Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis.
  • Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis. (en)
Title
  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.
  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase. (en)
skos:prefLabel
  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.
  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase. (en)
skos:notation
  • RIV/61389030:_____/03:56033097!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany
  • 63;66
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA301/02/0475), Z(AV0Z5038910), Z(MSM 153100008)
http://linked.open...iv/cisloPeriodika
  • 1/3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 602573
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/03:56033097
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Adenosine kinase; Tobacco Bright Yellow 2; Cytokinin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [20CB01950B2F]
http://linked.open...i/riv/nazevZdroje
  • FEBS Letters
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 533
http://linked.open...iv/tvurceVysledku
  • Strnad, Miroslav
  • Lenobel, René
  • Witters, E.
  • Onckelen, H.
  • Laukens, K.
http://linked.open...n/vavai/riv/zamer
issn
  • 0014-5793
number of pages
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