About: In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus.

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About: In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two types of phospholipid degrading enzyme, phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl- inositol-specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two types of activity; polyphosphoinositide-requiring (PIP2-dependent PLD) and polyphosphoinositide-independent requiring millimolar concentrations of calcium (PLD). Significantly different patterns of activity profiles were found for soluble and membrane-associated forms of all three enzymes within both processes. Membrane-associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD, while the highest PI-PLC activity appears in the same stages of development of seeds and plants as for PLD. In subcellular fractions of hypocotyls of young plants, phospholipases were localized predominantly on plasma membranes. The biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles, isolated by partitionin Two types of phospholipid degrading enzyme, phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl- inositol-specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two types of activity; polyphosphoinositide-requiring (PIP2-dependent PLD) and polyphosphoinositide-independent requiring millimolar concentrations of calcium (PLD). Significantly different patterns of activity profiles were found for soluble and membrane-associated forms of all three enzymes within both processes. Membrane-associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD, while the highest PI-PLC activity appears in the same stages of development of seeds and plants as for PLD. In subcellular fractions of hypocotyls of young plants, phospholipases were localized predominantly on plasma membranes. The biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles, isolated by partitionin (en)
Title	In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus. In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus. (en)
skos:prefLabel	In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus. In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus. (en)
skos:notation	RIV/61389030:_____/03:56033064!RIV/2004/GA0/A56004/N
http://linked.open.../vavai/riv/strany	691;698
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/00/1332), P(LN00A081), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	383
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Martinec, Jan Veselková, Štěpánka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	610058
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/03:56033064
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Brassica napus; phospholipases; plasma membrane (en)
http://linked.open.../riv/klicoveSlovo	Brassica napus plasma membrane phospholipases
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[413EC9C561FB]
http://linked.open...i/riv/nazevZdroje	JOURNAL OF EXPERIMENTAL BOTANY
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of phospholipid degrading in plant signal transduction Signaling pathways in plants
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Martinec, Jan Novotná, Z. Valentová, O. Kader, J. K. Profotová, B. Žďárová, Štěpánka
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0022-0957
number of pages	8 (xsd:int)

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