About: Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells.

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About: Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Changes in levels of endogenous cytokinins and activity of cytokinin oxidase (EC 1.4.3.6) were followed during the cell cycle progression in large-scale (ca. 30 g FW) synchronized culture of the tobacco cell line BY-2. Synchronization was induced either by 4 mM hydroxyurea or 15 iM aphidicolin alone (a single block of the cell cycle at G,/S) or in combination with 1 uM oryzalin (double blocking at G,/S and Gz/M). Up to 46% synchrony was obtained. Two distinct cytokinin peaks were detected at 0-2 h and 8.5-9 h after the release from the first block coinciding with the beginning of the S phase and with the onset of mitosis, respectively. Interestingly, as identified by MS/MS, very sharp and transient premitotic peak of cytokinins contained, in addition to fra/u-zeatin, also considerable amounts of cu-zeatin and zeatin-0-glucoside. Cytokinin oxidase activity exhibited conspicuous maximum parallel to the first peak of cytokinin accumulation and deep minimum corresponding to the culmination of mitotic inde Changes in levels of endogenous cytokinins and activity of cytokinin oxidase (EC 1.4.3.6) were followed during the cell cycle progression in large-scale (ca. 30 g FW) synchronized culture of the tobacco cell line BY-2. Synchronization was induced either by 4 mM hydroxyurea or 15 iM aphidicolin alone (a single block of the cell cycle at G,/S) or in combination with 1 uM oryzalin (double blocking at G,/S and Gz/M). Up to 46% synchrony was obtained. Two distinct cytokinin peaks were detected at 0-2 h and 8.5-9 h after the release from the first block coinciding with the beginning of the S phase and with the onset of mitosis, respectively. Interestingly, as identified by MS/MS, very sharp and transient premitotic peak of cytokinins contained, in addition to fra/u-zeatin, also considerable amounts of cu-zeatin and zeatin-0-glucoside. Cytokinin oxidase activity exhibited conspicuous maximum parallel to the first peak of cytokinin accumulation and deep minimum corresponding to the culmination of mitotic inde (en)
Title	Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. (en)
skos:prefLabel	Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells. (en)
skos:notation	RIV/61389030:_____/02:56023019!RIV/2003/GA0/A56003/N
http://linked.open.../vavai/riv/strany	333;337
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/99/1130), P(GV206/96/K188), P(IAA6038002), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Motyka, Václav Dobrev, Petre Trávníčková, Alena Vaňková, Radomíra Malbeck, Jiří Gaudinová, Alena Kamínek, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	667267
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/02:56023019
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cis zeatin; Cytokinins; Cytokinin oxidase (en)
http://linked.open.../riv/klicoveSlovo	Cytokinins Cis zeatin Cytokinin oxidase
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[A1BA75CDC16F]
http://linked.open...i/riv/nazevZdroje	Plant Physiology and Biochemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of glycosylation in regulation of cytokinin levels and control of morphogenic responses of selected crop plants Cytokinins and auxins in regulation of plant development Metabolic inactivation of cytokinins in plants and its physiological significance
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	40
http://linked.open...iv/tvurceVysledku	Dobrev, Petre Malbeck, Jiří Motyka, Václav Trávníčková, Alena Vaňková, Radomíra Gaudinová, Alena Kamínek, Miroslav
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0981-9428
number of pages	5 (xsd:int)

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