About: Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L).

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About: Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L). Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a high-purity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL-1RS transloca-tion, have been found and lines from which translocation chromosomes 5BL-7BL and 4AL-4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be used for sortin Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a high-purity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL-1RS transloca-tion, have been found and lines from which translocation chromosomes 5BL-7BL and 4AL-4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be used for sortin (en)
Title	Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L). Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L). (en)
skos:prefLabel	Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L). Flow kaiyotyping and chromosome sorting in bread wheat (Triticum aestivum L). (en)
skos:notation	RIV/61389030:_____/02:56023014!RIV/2003/AV0/A56003/N
http://linked.open.../vavai/riv/strany	1362;1372
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA6038204), P(IBS5038104), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Doležel, Jaroslav Šimková, Hana Kubaláková, Marie Číhalíková, Jarmila Vrána, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	646340
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/02:56023014
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chromosome isolation; Chromosome sorting; Flow cytometry (en)
http://linked.open.../riv/klicoveSlovo	Chromosome isolation Chromosome sorting Flow cytometry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[E2A4CE7F59F9]
http://linked.open...i/riv/nazevZdroje	Theoretical and Applied Genetics
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Construction of banana bacterial artificial chromosome (BAC) library for physical genome mapping Molecular cytogenetic and cytometric methods for breeding of grasses and trifolium
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	104
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Šimková, Hana Doleželová, Marie Vrána, Jan Čihalíková, Jarmila
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0040-5752
number of pages	11 (xsd:int)

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