About: Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction.

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About: Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A method for separation of cytokinins from auxin and abscisic acid, which allows further separation ofcylokinin ribotides from cytokinin bases, ribosidcs und glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationie cylokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluled by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of (he method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (R1A) or I1PLC-MS. A method for separation of cytokinins from auxin and abscisic acid, which allows further separation ofcylokinin ribotides from cytokinin bases, ribosidcs und glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationie cylokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluled by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of (he method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (R1A) or I1PLC-MS. (en)
Title	Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. (en)
skos:prefLabel	Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction. (en)
skos:notation	RIV/61389030:_____/02:56023005!RIV/2003/GA0/A56003/N
http://linked.open.../vavai/riv/strany	21;29
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/02/0967), P(GA522/02/0530), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Dobrev, Petre Kamínek, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	646019
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/02:56023005
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytokinins; abscisic acid; extraction (en)
http://linked.open.../riv/klicoveSlovo	extraction abscisic acid cytokinins
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[3C302FE35EE8]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Hormonal regulation of leaf senescence and its relation to utilization of nitrogen in cereals Targeting of cytokinin oxidase and its role in regulation of plant development
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	950
http://linked.open...iv/tvurceVysledku	Dobrev, Petre Kamínek, Miroslav
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0021-9673
number of pages	9 (xsd:int)

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