About: DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage.

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About: DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The level of hydrogen peroxide (HaOaMnduced genomic DNA damage measured by the Comet assay in tobacco suspension cells (TX1) increased as a function of the age of the culture. After treatment of TX1 cells with 15 mM H2O2, the average ( S.E.) median tail moment value was only 4.85 1.00 \|xm in nuclei isolated from 2-day-old cells compared to 72.33 1.40 (xm in nuclei isolated from 12-day-old cells. By contrast, nuclei first isolated from 2 and 12-day-old cells and then treated with HfeC^, expressed the same level of DNA damage. The activity of catalases was markedly higher in 2-day-old TX1 cells compared to 12-day-old cells. The results indicate that the reaction of the H2O2 with nuclear DNA is modified by the presence of the plant cell wall, and enzymes and macromolecules present in the cytosol, and is not connected with changes in the nuclear DNA sensitivity during cell suspension growth. ř 2002 Elsevier Science B.V. All rights reserved. The level of hydrogen peroxide (HaOaMnduced genomic DNA damage measured by the Comet assay in tobacco suspension cells (TX1) increased as a function of the age of the culture. After treatment of TX1 cells with 15 mM H2O2, the average ( S.E.) median tail moment value was only 4.85 1.00 \|xm in nuclei isolated from 2-day-old cells compared to 72.33 1.40 (xm in nuclei isolated from 12-day-old cells. By contrast, nuclei first isolated from 2 and 12-day-old cells and then treated with HfeC^, expressed the same level of DNA damage. The activity of catalases was markedly higher in 2-day-old TX1 cells compared to 12-day-old cells. The results indicate that the reaction of the H2O2 with nuclear DNA is modified by the presence of the plant cell wall, and enzymes and macromolecules present in the cytosol, and is not connected with changes in the nuclear DNA sensitivity during cell suspension growth. ř 2002 Elsevier Science B.V. All rights reserved. (en)
Title	DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage. DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage. (en)
skos:prefLabel	DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage. DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage. (en)
skos:notation	RIV/61389030:_____/02:56023001!RIV/2003/GA0/A56003/N
http://linked.open.../vavai/riv/strany	147;152
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/02/0400), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Gichner, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	643496
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/02:56023001
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA; peroxide; tobacco (en)
http://linked.open.../riv/klicoveSlovo	DNA peroxide tobacco
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[63412E3CCD17]
http://linked.open...i/riv/nazevZdroje	Mutation Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Genotoxicity of environmental mutagens in higher plants detected by molecular biological methods
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	514
http://linked.open...iv/tvurceVysledku	Gichner, Tomáš Stavreva, D.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	1383-5718
number of pages	6 (xsd:int)

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