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|---|
| rdf:type
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| Description
| - Monodisperse macroporous poly(glycidyl methacrylate) (PGMA) microspheres were prepared by multiple swelling polymerization using a modified Ugelstad method. The outer and inner parts of PGMA microspheres were coated with silica (SiO2) using tetramethyl othosilicate (TMOS) and trimethoxy(methyl)silane (TMMS) to produce PGMA&SiO2 and PGMA&Me-SiO2 hybrid particles, respectively. In order to introduce amino groups, the microsphere surface was functionalized with (3-aminopropyl)diethoxy(methyl)silane (APDMS) and (3-aminopropyl)triethoxysilane (APTES) resulting in PGMA&SiO2-NH2 and PGMA&Me-SiO2-NH2 particles, respectively. The particles were characterized by SEM and TEM coupled with energy dispersive X-ray analysis (EDAX) and FT-IR spectroscopy to determine morphology, particle size, polydispersity and the presence of functional groups. Elemental and thermogravimetric (TGA) analyses were used for determination of the formed SiO2. Moreover, specific surface areas of the hybrid microspheres were determined by dynamic adsorption of nitrogen. In biological experiments, PGMA, PGMA&SiO2 and PGMA&SiO2-NH2 microspheres demonstrated good biocompatibility in vitro, since no significant increase in the amount of dead (Trypan Blue) human Jurkat T cells was observed when the microsphere concentrations in the medium increased from 0.1 to 1 μg/ml. The microspheres exhibited red and green fluorescence. The former increased in the order PGMA <PGMA&SiO2< PGMA&SiO2-NH2. The PGMA&SiO2-NH2 microspheres did not significantly swell at pH 7.0–9.5. They were capable of binding biomacromolecules, e.g., bovine serum albumin by adsorption or Dextran Blue by covalent binding.
- Monodisperse macroporous poly(glycidyl methacrylate) (PGMA) microspheres were prepared by multiple swelling polymerization using a modified Ugelstad method. The outer and inner parts of PGMA microspheres were coated with silica (SiO2) using tetramethyl othosilicate (TMOS) and trimethoxy(methyl)silane (TMMS) to produce PGMA&SiO2 and PGMA&Me-SiO2 hybrid particles, respectively. In order to introduce amino groups, the microsphere surface was functionalized with (3-aminopropyl)diethoxy(methyl)silane (APDMS) and (3-aminopropyl)triethoxysilane (APTES) resulting in PGMA&SiO2-NH2 and PGMA&Me-SiO2-NH2 particles, respectively. The particles were characterized by SEM and TEM coupled with energy dispersive X-ray analysis (EDAX) and FT-IR spectroscopy to determine morphology, particle size, polydispersity and the presence of functional groups. Elemental and thermogravimetric (TGA) analyses were used for determination of the formed SiO2. Moreover, specific surface areas of the hybrid microspheres were determined by dynamic adsorption of nitrogen. In biological experiments, PGMA, PGMA&SiO2 and PGMA&SiO2-NH2 microspheres demonstrated good biocompatibility in vitro, since no significant increase in the amount of dead (Trypan Blue) human Jurkat T cells was observed when the microsphere concentrations in the medium increased from 0.1 to 1 μg/ml. The microspheres exhibited red and green fluorescence. The former increased in the order PGMA <PGMA&SiO2< PGMA&SiO2-NH2. The PGMA&SiO2-NH2 microspheres did not significantly swell at pH 7.0–9.5. They were capable of binding biomacromolecules, e.g., bovine serum albumin by adsorption or Dextran Blue by covalent binding. (en)
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| Title
| - Novel fluorescent poly(glycidyl methacrylate) - silica microspheres
- Novel fluorescent poly(glycidyl methacrylate) - silica microspheres (en)
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| skos:prefLabel
| - Novel fluorescent poly(glycidyl methacrylate) - silica microspheres
- Novel fluorescent poly(glycidyl methacrylate) - silica microspheres (en)
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| skos:notation
| - RIV/61389013:_____/14:00427979!RIV15-AV0-61389013
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(ED1.1.00/02.0109), P(EE2.3.30.0029)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389013:_____/14:00427979
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - microspheres; silica; poly(glycidyl methacrylate) (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Horák, Daniel
- Boiko, N.
- Klyuchivska, O.
- Stoika, R.
- Grama, Silvia
- Bilyy, R.
- Antonyuk, V.
|
| http://linked.open...ain/vavai/riv/wos
| |
| issn
| |
| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1016/j.eurpolymj.2014.04.011
|
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