About: A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection

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About: A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetic bead cellulose (MBC) was prepared using sol–gel transition of viscose in the presence of maghemite (γ-Fe2O3) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA. Magnetic bead cellulose (MBC) was prepared using sol–gel transition of viscose in the presence of maghemite (γ-Fe2O3) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA. (en)
Title	A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection (en)
skos:prefLabel	A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection (en)
skos:notation	RIV/61389013:_____/13:00394938!RIV14-MSM-61389013
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(7E12054), P(GAP503/10/0664)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Lenfeld, Jiří Horák, Daniel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	59162
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/13:00394938
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bead cellulose; magnetic; protein detection (en)
http://linked.open.../riv/klicoveSlovo	bead cellulose protein detection magnetic
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E00C48C1DB08]
http://linked.open...i/riv/nazevZdroje	Journal of Biotechnology
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Tools for minimally invasive diagnostics Isolation, typing and the development of immunochemical and instrumetal methods for detection and characterization of Cronobacter sp.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	167
http://linked.open...iv/tvurceVysledku	Horák, Daniel Yan, J. Lenfeld, Jiří Hammond, M. Kamali-Moghaddam, M.
http://linked.open...ain/vavai/riv/wos	000324040300005
issn	0168-1656
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.jbiotec.2013.06.010

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