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| rdf:type
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| Description
| - Maghemite (γ-Fe2O3) nanoparticles were prepared by coprecipitation of Fe(II) and Fe(III) salts followed by oxidation of Fe3O4 with sodium hypochlorite. The γ-Fe2O3 nanoparticles were then coated by a silica shell using tetramethyl orthosilicate producing γ-Fe2O3@SiO2 particles. In order to introduce amino groups, the surface of the γ-Fe2O3@SiO2 particles was coated with a second silica shell using (3-aminopropyl)triethoxysilane, resulting in γ-Fe2O3@SiO2-NH2 nanoparticles. The particles were characterized by scanning and transmission electron microscopy (SEM and TEM), dynamic light scattering (DLS) and Fourier-transform infrared (FT-IR) spectroscopy in terms of determination of morphology, particle size, polydispersity and presence of functional groups. Atomic absorption spectroscopy (AAS) was used for determination of the iron content. Moreover, induced magnetization and temperature-dependent magnetic susceptibility were investigated. In the biological experiments, γ-Fe2O3@SiO2 and γ-Fe2O3@SiO2-NH2 nanoparticles were incubated with murine macrophages of J774.2 line and the uptake of particles by the cells was visualized using both light and fluorescence microscopy. The silica surface coating did not affect the nanoparticle uptake by the cells, however, it decreased induced cytoplasmic vacuolization of macrophages.
- Maghemite (γ-Fe2O3) nanoparticles were prepared by coprecipitation of Fe(II) and Fe(III) salts followed by oxidation of Fe3O4 with sodium hypochlorite. The γ-Fe2O3 nanoparticles were then coated by a silica shell using tetramethyl orthosilicate producing γ-Fe2O3@SiO2 particles. In order to introduce amino groups, the surface of the γ-Fe2O3@SiO2 particles was coated with a second silica shell using (3-aminopropyl)triethoxysilane, resulting in γ-Fe2O3@SiO2-NH2 nanoparticles. The particles were characterized by scanning and transmission electron microscopy (SEM and TEM), dynamic light scattering (DLS) and Fourier-transform infrared (FT-IR) spectroscopy in terms of determination of morphology, particle size, polydispersity and presence of functional groups. Atomic absorption spectroscopy (AAS) was used for determination of the iron content. Moreover, induced magnetization and temperature-dependent magnetic susceptibility were investigated. In the biological experiments, γ-Fe2O3@SiO2 and γ-Fe2O3@SiO2-NH2 nanoparticles were incubated with murine macrophages of J774.2 line and the uptake of particles by the cells was visualized using both light and fluorescence microscopy. The silica surface coating did not affect the nanoparticle uptake by the cells, however, it decreased induced cytoplasmic vacuolization of macrophages. (en)
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| Title
| - Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages
- Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages (en)
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| skos:prefLabel
| - Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages
- Silica-coated .gamma.-Fe2O3 nanoparticles: preparation and engulfment by mammalian macrophages (en)
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| skos:notation
| - RIV/61389013:_____/13:00394385!RIV14-MSM-61389013
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(7E12054), P(GCP207/12/J013)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389013:_____/13:00394385
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - maghemite; nanoparticles; silica (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Nanopharmaceutics and Drug Delivery
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Horák, Daniel
- Trchová, Miroslava
- Petrovský, Eduard
- Boiko, N.
- Klyuchivska, O.
- Stoika, R.
- Zasońska, Beata Anna
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| |
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