About: DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics

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About: DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Structural changes occurring on a millisecond time scale during uptake of DNA by cationic lipid nanocarriers are monitored by time-resolved small-angle X-ray scattering (SAXS) coupled to a rapid-mixing stopped-flow technique. Nanoparticles (NPs) of nanochannel organization are formed by PEGylation, hydration, and dispersion of a lipid film of the fusogenic lipid monoolein in a mixture with positively charged (DOMA) and PEGylated (DOPE-PEG2000) amphiphiles and are characterized by the inner cubic structure of very large nanochannels favorable for DNA upload. Ultrafast structural dynamics of complexation and assembly of these cubosome particles with neurotrophic plasmid DNA (pDNA) is revealed thanks to the high brightness of the employed synchrotron X-ray beam. The rate constant of the pDNA/lipid NP complexation is estimated from dynamic roentgenograms recorded at 4 ms time resolution. pDNA upload into the vastly hydrated channels of the cubosome carriers leads to a fast nanoparticle–nanoparticle structural transition and lipoplex formation involving tightly packed pDNA. Structural changes occurring on a millisecond time scale during uptake of DNA by cationic lipid nanocarriers are monitored by time-resolved small-angle X-ray scattering (SAXS) coupled to a rapid-mixing stopped-flow technique. Nanoparticles (NPs) of nanochannel organization are formed by PEGylation, hydration, and dispersion of a lipid film of the fusogenic lipid monoolein in a mixture with positively charged (DOMA) and PEGylated (DOPE-PEG2000) amphiphiles and are characterized by the inner cubic structure of very large nanochannels favorable for DNA upload. Ultrafast structural dynamics of complexation and assembly of these cubosome particles with neurotrophic plasmid DNA (pDNA) is revealed thanks to the high brightness of the employed synchrotron X-ray beam. The rate constant of the pDNA/lipid NP complexation is estimated from dynamic roentgenograms recorded at 4 ms time resolution. pDNA upload into the vastly hydrated channels of the cubosome carriers leads to a fast nanoparticle–nanoparticle structural transition and lipoplex formation involving tightly packed pDNA. (en)
Title	DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics (en)
skos:prefLabel	DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics DNA/Fusogenic lipid nanocarrier assembly: millisecond structural dynamics (en)
skos:notation	RIV/61389013:_____/13:00392998!RIV14-GA0-61389013
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP208/10/1600)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Štěpánek, Petr Filippov, Sergey Angelov, Borislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	70160
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/13:00392998
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lipoplex; SAXS; cryo-TEM (en)
http://linked.open.../riv/klicoveSlovo	SAXS cryo-TEM lipoplex
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[53F3F8CA6C85]
http://linked.open...i/riv/nazevZdroje	Journal of Physical Chemistry Letters
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Polymeric particles and nanostructured materials stabilized by surface active molecules
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Filippov, Sergey Štěpánek, Petr Angelov, Borislav Angelova, A. Couvreur, P. Lesieur, S. Drechsler, M. Narayanan, T.
http://linked.open...ain/vavai/riv/wos	000320214600034
issn	1948-7185
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/jz400857z

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