About: Polymer-modified gene delivery vectors retargeted with recombinant proteins

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.appleacademicpress.com/title.php?id=9781926895178
Description	We have prepared and characterized a multivalent reactive N-(2-hydroxypropyl)methacrylamide copolymer (PHPMA) bearing bungarotoxin-binding peptide (BTXbp). The polymer was used for covalent surface modification of adenoviral vectors containing luciferase reporter gene (Ad). The peptide BTXbp is known to have extremely high binding affinity to bungarotoxin (BTX) –a protein strongly binding to acetylcholine receptors. A recombinant protein consisting of antiPSMA antibody scFv fragment and BTX binding site (BTX-scFv) was used for retargeting of the polymer-modified Ad to prostate-specific membrane antigen (PSMA) receptors. While the polymer-modified Ad exhibited approximately 100-fold lower infectivity than the naked virus (in terms of luciferase expression), the addition of BTX-scFv to the polymer-coated Ad led to about 10-fold restoration of luciferase expression in PSMA-positive LNCaP cells. No such increase of transduction activity was observed in PSMA-negative PC3 cells. We have shown that the presented PHPMA-BTXbp/BTX-scFv system can be used as a universal tool for a receptor-specific viral gene therapy. We have prepared and characterized a multivalent reactive N-(2-hydroxypropyl)methacrylamide copolymer (PHPMA) bearing bungarotoxin-binding peptide (BTXbp). The polymer was used for covalent surface modification of adenoviral vectors containing luciferase reporter gene (Ad). The peptide BTXbp is known to have extremely high binding affinity to bungarotoxin (BTX) –a protein strongly binding to acetylcholine receptors. A recombinant protein consisting of antiPSMA antibody scFv fragment and BTX binding site (BTX-scFv) was used for retargeting of the polymer-modified Ad to prostate-specific membrane antigen (PSMA) receptors. While the polymer-modified Ad exhibited approximately 100-fold lower infectivity than the naked virus (in terms of luciferase expression), the addition of BTX-scFv to the polymer-coated Ad led to about 10-fold restoration of luciferase expression in PSMA-positive LNCaP cells. No such increase of transduction activity was observed in PSMA-negative PC3 cells. We have shown that the presented PHPMA-BTXbp/BTX-scFv system can be used as a universal tool for a receptor-specific viral gene therapy. (en)
Title	Polymer-modified gene delivery vectors retargeted with recombinant proteins Polymer-modified gene delivery vectors retargeted with recombinant proteins (en)
skos:prefLabel	Polymer-modified gene delivery vectors retargeted with recombinant proteins Polymer-modified gene delivery vectors retargeted with recombinant proteins (en)
skos:notation	RIV/61389013:_____/12:00381465!RIV13-AV0-61389013
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0505), P(GA203/08/0543), Z(AV0Z40500505)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Ulbrich, Karel Pechar, Michal Pola, Robert
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	159559
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/12:00381465
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bungarotoxin; poly(ethylene glycol); prostate-specific membrane antigen (en)
http://linked.open.../riv/klicoveSlovo	bungarotoxin poly(ethylene glycol) prostate-specific membrane antigen
http://linked.open...ontrolniKodProRIV	[FBE7CB40BB2D]
http://linked.open...i/riv/mistoVydani	Oakville
http://linked.open...vEdiceCisloSvazku	Advances in Nanoscience and Nanotechnology
http://linked.open...i/riv/nazevZdroje	Nanomedicine and Drug Delivery
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	320 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs Hybrid macromolecular systems based on hydrophilic polymers and stimuli-responsive peptides
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Pechar, Michal Pola, Robert Ulbrich, Karel Seymour, L. W. Carlisle, R. C. Willemsen, R. A.
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Apple Academic Press
https://schema.org/isbn	978-1-926895-17-8

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