About: Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study

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About: Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The entrapment of protein molecules in cubosomic nanocarriers that are sterically stabilized by an amphiphilic poly(ethylene glycol) (PEG) derivative are investigated. The mechanism of fragmentation of a self-assembled PEGylated cubic lipid phase into nanoparticles (NPs) is studied in excess aqueous medium. The molar ratio between the cubic-phase-forming lipid monoolein (MO) and its PEGylated derivative (MOPEG2000) is selected as to favor the formation of inverted-type liquid-crystalline (LC) structures. Freeze-fracture electron microscopy (FF-EM), quasi-elastic light scattering (QELS), confocal laser scanning fluorescence microscopy (CLSFM) and far-UV synchrotron radiation circular dichroism (SRCD) spectroscopy are applied for determination of the NPs' sizes, inner organization, stability, and interaction with the protein α-chymotrypsinogen A. The PEGylated cubosomes offer new possibilities for investigation of protein loading in sterically stabilized (“Stealth) lipid carriers. The entrapment of protein molecules in cubosomic nanocarriers that are sterically stabilized by an amphiphilic poly(ethylene glycol) (PEG) derivative are investigated. The mechanism of fragmentation of a self-assembled PEGylated cubic lipid phase into nanoparticles (NPs) is studied in excess aqueous medium. The molar ratio between the cubic-phase-forming lipid monoolein (MO) and its PEGylated derivative (MOPEG2000) is selected as to favor the formation of inverted-type liquid-crystalline (LC) structures. Freeze-fracture electron microscopy (FF-EM), quasi-elastic light scattering (QELS), confocal laser scanning fluorescence microscopy (CLSFM) and far-UV synchrotron radiation circular dichroism (SRCD) spectroscopy are applied for determination of the NPs' sizes, inner organization, stability, and interaction with the protein α-chymotrypsinogen A. The PEGylated cubosomes offer new possibilities for investigation of protein loading in sterically stabilized (“Stealth) lipid carriers. (en)
Title	Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study (en)
skos:prefLabel	Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study Protein-containing PEGylated cubosomic particles: freeze-fracture electron microscopy and synchrotron radiation circular dichroism study (en)
skos:notation	RIV/61389013:_____/12:00378735!RIV13-AV0-61389013
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GAP208/10/1600), Z(AV0Z40500505)
http://linked.open...iv/cisloPeriodika	26
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Angelov, Borislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	163060
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/12:00378735
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	freeze-fracture electron microscopy; synchrotron radiation circular dichroism; SAXS (en)
http://linked.open.../riv/klicoveSlovo	SAXS freeze-fracture electron microscopy synchrotron radiation circular dichroism
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C69F53C4FD7E]
http://linked.open...i/riv/nazevZdroje	Journal of Physical Chemistry B
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Polymeric particles and nanostructured materials stabilized by surface active molecules
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	116
http://linked.open...iv/tvurceVysledku	Angelov, Borislav Angelova, A. Lesieur, S. Nicolas, V. Hoffmann, S. V. Papahadjopoulos-Sternberg, B.
http://linked.open...ain/vavai/riv/wos	000305933800009
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
issn	1520-6106
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/jp303863q

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