About: Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans. The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans. (en)
Title	Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy (en)
skos:prefLabel	Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy (en)
skos:notation	RIV/61389013:_____/11:00367418!RIV12-AV0-61389013
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP205/10/0348), P(KAN200520704), P(KJB600960906), Z(AV0Z40500505), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Šlouf, Miroslav Pavlová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	186749
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/11:00367418
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	electron microscopy; colloidal palladium; nanoparticles; labeling; salivary glands; Ixodes ricinus (en)
http://linked.open.../riv/klicoveSlovo	Ixodes ricinus colloidal palladium electron microscopy labeling salivary glands nanoparticles
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[AED77C24F9F7]
http://linked.open...i/riv/nazevZdroje	Microscopy and Microanalysis
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Sandwich nanocomposites: preparation, nucleation effects, electrical properties. Glycoprotein analysis of Ixodes ricinus salivary glands New nanoparticles for ultrastructural diagnostics
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Langhans, Jan Nebesářová, Jana Pavlová, Eva Vancová, Marie Šlouf, Miroslav
http://linked.open...ain/vavai/riv/wos	000295609100020
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	1431-9276
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1017/S1431927611000547

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 67 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software