About: Removable nanocoatings for siRNA polyplexes

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Intracellularly removable polymer nanocoatings designed for surface modification of siRNA-based polyplexes (PEC) were developed with the aim to improve properties of PEC in physiological environment. N-(2-hydroxypropyl)methacrylamide (HPMA) based copolymers containing reactive groups bound via biodegradable disulfide spacers to the polymeric backbone were synthesized and used for coating of PEC formed by the self-assembly of siRNA with the polycations polyethylene imine and poly(HPMA)-grafted poly(l-lysine) (GPL). Light scattering analysis showed the attachment of, on average, 28 polymer molecules to the surface of the polyplexes forming a 5-nm-thick hydrophilic stealth coating. Coating of the siRNA/GPL PEC substantially improved their stability in physiological solutions and an initial proof-of-principle for time- and concentration-dependent specific gene silencing was obtained. It was demonstrated that siRNA/GPL complexes hold significant potential for further development. Intracellularly removable polymer nanocoatings designed for surface modification of siRNA-based polyplexes (PEC) were developed with the aim to improve properties of PEC in physiological environment. N-(2-hydroxypropyl)methacrylamide (HPMA) based copolymers containing reactive groups bound via biodegradable disulfide spacers to the polymeric backbone were synthesized and used for coating of PEC formed by the self-assembly of siRNA with the polycations polyethylene imine and poly(HPMA)-grafted poly(l-lysine) (GPL). Light scattering analysis showed the attachment of, on average, 28 polymer molecules to the surface of the polyplexes forming a 5-nm-thick hydrophilic stealth coating. Coating of the siRNA/GPL PEC substantially improved their stability in physiological solutions and an initial proof-of-principle for time- and concentration-dependent specific gene silencing was obtained. It was demonstrated that siRNA/GPL complexes hold significant potential for further development. (en)
Title	Removable nanocoatings for siRNA polyplexes Removable nanocoatings for siRNA polyplexes (en)
skos:prefLabel	Removable nanocoatings for siRNA polyplexes Removable nanocoatings for siRNA polyplexes (en)
skos:notation	RIV/61389013:_____/11:00356597!RIV11-AV0-61389013
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAAX00500803), P(KAN200200651), Z(AV0Z40500505)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Ulbrich, Karel Špírková, Milena Šubr, Vladimír Koňák, Čestmír Kostka, Libor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav makromolekulární chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	226520
http://linked.open...ai/riv/idVysledku	RIV/61389013:_____/11:00356597
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	polyelectrolyte complexes; siRNA; nanocoating (en)
http://linked.open.../riv/klicoveSlovo	siRNA nanocoating polyelectrolyte complexes
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[DF3C195712B7]
http://linked.open...i/riv/nazevZdroje	Bioconjugate Chemistry
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Nanoparticulate and supramolecular systems for targeted drug delivery Multifunctional Polymer Systems with Cytostatic and Immunomobilizing Activities
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	22
http://linked.open...iv/tvurceVysledku	Ulbrich, Karel Špírková, Milena Šubr, Vladimír Koňák, Čestmír Addadi, Y. Kostka, Libor Lammers, T. Neeman, M.
http://linked.open...ain/vavai/riv/wos	000287297000009
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie
issn	1043-1802
number of pages	11 (xsd:int)

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