About: Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to compare two methods for quantification of changes in intracellular potassium concentration (decrease from similar to 140 to similar to 20 mM) due to the action of a pore-forming toxin, the adenylate cyclase toxin (CyaA) from the pathogenic bacterium Bordetella pertussis. CyaA was incubated with stably transfected K1 Chinese hamster ovary cells expressing the toxin receptor CD11b/CD18 and the decrease in potassium concentration in the cells was followed by inductively coupled plasma mass spectrometry (ICP-MS). It is shown that this method is superior in terms of sensitivity, accuracy, and temporal resolution over the method employing the potassium-binding benzofuran isophthalate-acetoxymethyl ester fluorescent indicator. The ICP-MS procedure was found to be a reliable and straightforward analytical approach enabling kinetic studies of CyaA action at physiologically relevant toxin concentrations (<1000 ng/ml) in biological microsamples The aim of this study was to compare two methods for quantification of changes in intracellular potassium concentration (decrease from similar to 140 to similar to 20 mM) due to the action of a pore-forming toxin, the adenylate cyclase toxin (CyaA) from the pathogenic bacterium Bordetella pertussis. CyaA was incubated with stably transfected K1 Chinese hamster ovary cells expressing the toxin receptor CD11b/CD18 and the decrease in potassium concentration in the cells was followed by inductively coupled plasma mass spectrometry (ICP-MS). It is shown that this method is superior in terms of sensitivity, accuracy, and temporal resolution over the method employing the potassium-binding benzofuran isophthalate-acetoxymethyl ester fluorescent indicator. The ICP-MS procedure was found to be a reliable and straightforward analytical approach enabling kinetic studies of CyaA action at physiologically relevant toxin concentrations (<1000 ng/ml) in biological microsamples (en)
Title	Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin (en)
skos:prefLabel	Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin Quantification of potassium levels in cells treated with Bordetella adenylate cyclase toxin (en)
skos:notation	RIV/61388971:_____/14:00428410!RIV15-GA0-61388971
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-14547S), P(GAP302/11/0580), P(GAP302/12/0460), P(GPP207/12/P890)
http://linked.open...iv/cisloPeriodika	APR 2014
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Osička, Radim Wald, Tomáš Šebo, Peter Mašín, Jiří Fišer, Radovan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	41069
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/14:00428410
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Potassium; Adenylate cyclase toxin; RTX (en)
http://linked.open.../riv/klicoveSlovo	RTX Potassium Adenylate cyclase toxin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C24E026F049D]
http://linked.open...i/riv/nazevZdroje	Analytical Biochemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Interaction of adenylate cyclase toxin with complement receptor 3 Role of the pore-forming activity in adenylate cyclase toxin action and Bordetella virulence In vivo inkorporace fluorescenčních aminokyselin a ab initio modelování proteinů; univerzální nástroje pro zjištění topologie membránových proteinů. Mechanisms of immunomodulatory action of Bordetella pertussis virulence factors
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	450
http://linked.open...iv/tvurceVysledku	Matoušek, Tomáš Osička, Radim Wald, Tomáš Dědina, Jiří Šebo, Peter Fišer, Radovan Mašín, Jiří Petry-Podgorska, Inga
http://linked.open...ain/vavai/riv/wos	000333500900011
issn	0003-2697
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ab.2013.10.039

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