About: Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images

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About: Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The determination of phycobilisome diffusivity (diffusion coefficient D) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments is usually done by analytical models. However, analytical models need some unrealistic conditions to be supposed. This study describes the development of a method based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an objective function representing the disparity between the experimental and simulated time-varying concentration profiles. Our method improves on other models by accounting for experimentally measured time-varying Dirichlet boundary conditions, and can include a reaction term as well. As a result we obtain both the overall (time averaged) diffusion coefficient D and the sequence of diffusivities Dj based on two successive fluorescence profiles in j-th time interval. Due to the noisy data, we cope with an inverse ill-posed problem and a regularization technique is mandatory. The determination of phycobilisome diffusivity (diffusion coefficient D) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments is usually done by analytical models. However, analytical models need some unrealistic conditions to be supposed. This study describes the development of a method based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an objective function representing the disparity between the experimental and simulated time-varying concentration profiles. Our method improves on other models by accounting for experimentally measured time-varying Dirichlet boundary conditions, and can include a reaction term as well. As a result we obtain both the overall (time averaged) diffusion coefficient D and the sequence of diffusivities Dj based on two successive fluorescence profiles in j-th time interval. Due to the noisy data, we cope with an inverse ill-posed problem and a regularization technique is mandatory. (en)
Title	Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images (en)
skos:prefLabel	Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images (en)
skos:notation	RIV/61388971:_____/13:00425117!RIV14-MSM-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(ED2.1.00/03.0110), P(GA201/09/1957), P(GP206/09/P094), Z(AV0Z10300504), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	7-8
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kaňa, Radek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	73268
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/13:00425117
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	parameter estimation; FRAP; boundary value problem; optimization (en)
http://linked.open.../riv/klicoveSlovo	boundary value problem optimization parameter estimation FRAP
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[6B8D9D7FDF8E]
http://linked.open...i/riv/nazevZdroje	Mathematical and Computer Modelling
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Algal Biotechnology Třeboň (Algatech) South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Development of methods for solving large scale nonlinear programming and nonsmooth optimization problems Regulation of the non-photochemical quenching and light photosynthetic reactions - the role of ion fluxes in the cell and in the chloroplast
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Matonoha, Ctirad Papáček, Š. Kaňa, Radek
http://linked.open...ain/vavai/riv/wos	000315863300045
http://linked.open...n/vavai/riv/zamer	Informatika pro informační společnost: modely, algoritmy, aplikace Microorganisms in Research and Biotechnology
issn	0895-7177
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.mcm.2011.12.029

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