About: Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase     Goto   Sponge   NotDistinct   Permalink

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  • Production of the lignocellulose-degrading enzymes endo-1,4-beta-glucanase, 1,4-beta-glucosidase, cellobiohydrolase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, Mn peroxidase, and laccase was characterized in a common wood-rotting fungus Fomes fomentarius, a species able to efficiently decompose dead wood, and compared to the production in eight other fungal species. The main aim of this study was to characterize the 1,4-beta-glucosidase produced by F. fomentarius that was produced in high quantities in liquid stationary culture (25.9 U ml(-1)), at least threefold compared to other saprotrophic basidiomycetes, such as Rhodocollybia butyracea, Hypholoma fasciculare, Irpex lacteus, Fomitopsis pinicola, Pleurotus ostreatus, Piptoporus betulinus, and Gymnopus sp. (between 0.7 and 7.9 U ml(-1)). The 1,4-beta-glucosidase enzyme was purified to electrophoretic homogeneity by both anion-exchange and size-exclusion chromatography
  • Production of the lignocellulose-degrading enzymes endo-1,4-beta-glucanase, 1,4-beta-glucosidase, cellobiohydrolase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, Mn peroxidase, and laccase was characterized in a common wood-rotting fungus Fomes fomentarius, a species able to efficiently decompose dead wood, and compared to the production in eight other fungal species. The main aim of this study was to characterize the 1,4-beta-glucosidase produced by F. fomentarius that was produced in high quantities in liquid stationary culture (25.9 U ml(-1)), at least threefold compared to other saprotrophic basidiomycetes, such as Rhodocollybia butyracea, Hypholoma fasciculare, Irpex lacteus, Fomitopsis pinicola, Pleurotus ostreatus, Piptoporus betulinus, and Gymnopus sp. (between 0.7 and 7.9 U ml(-1)). The 1,4-beta-glucosidase enzyme was purified to electrophoretic homogeneity by both anion-exchange and size-exclusion chromatography (en)
Title
  • Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase
  • Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase (en)
skos:prefLabel
  • Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase
  • Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase (en)
skos:notation
  • RIV/61388971:_____/13:00423807!RIV14-MSM-61388971
http://linked.open...avai/riv/aktivita
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  • I, P(LA10001), P(OC08050)
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  • 1
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  • 74418
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  • RIV/61388971:_____/13:00423807
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  • Cellulose; 1,4-beta-glucosidase; Glycosyl hydrolase (en)
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  • US - Spojené státy americké
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  • [718B02EC82AC]
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  • Applied Biochemistry and Biotechnology
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  • 169
http://linked.open...iv/tvurceVysledku
  • Baldrian, Petr
  • Gabriel, Jiří
  • Větrovský, Tomáš
http://linked.open...ain/vavai/riv/wos
  • 000314023100009
issn
  • 0273-2289
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s12010-012-9952-9
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