About: Regulation of photosynthesis during heterocyst differentiation in Anabaena sp strain PCC 7120 investigated in vivo at single-cell level by chlorophyll fluorescence kinetic microscopy     Goto   Sponge   NotDistinct   Permalink

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  • Changes of photosynthetic activity in vivo of individual heterocysts and vegetative cells in the diazotrophic cyanobacterium Anabaena sp. strain PCC 7120 during the course of diazotrophic acclimation were determined using fluorescence kinetic microscopy (FKM). Distinct phases of stress and acclimation following nitrogen step-down were observed. The first was a period of perception, in which the cells used their internally stored nitrogen without detectable loss of PS II activity or pigments. In the second, the stress phase of nitrogen limitation, the cell differentiation occurred and an abrupt decline of fluorescence yield was observed. This decline in fluorescence was not paralleled by a corresponding decline in photosynthetic pigment content and PS II activity. Both maximal quantum yield and sustained electron flow were not altered in vegetative cells, only in the forming heterocysts. The third, acclimation phase started first in the differentiating heterocysts with a recovery of PS II photochemical yields Afterwards, the onset of nitrogenase activity was observed, followed by the restoration of antenna pigments in the vegetative cells, but not in the heterocysts. Surprisingly, mature heterocysts were found to have an intact PS II as judged by photochemical yields, but a strongly reduced PS II-associated antenna as judged by decreased F (0). The possible importance of the functional PS II in heterocysts is discussed
  • Changes of photosynthetic activity in vivo of individual heterocysts and vegetative cells in the diazotrophic cyanobacterium Anabaena sp. strain PCC 7120 during the course of diazotrophic acclimation were determined using fluorescence kinetic microscopy (FKM). Distinct phases of stress and acclimation following nitrogen step-down were observed. The first was a period of perception, in which the cells used their internally stored nitrogen without detectable loss of PS II activity or pigments. In the second, the stress phase of nitrogen limitation, the cell differentiation occurred and an abrupt decline of fluorescence yield was observed. This decline in fluorescence was not paralleled by a corresponding decline in photosynthetic pigment content and PS II activity. Both maximal quantum yield and sustained electron flow were not altered in vegetative cells, only in the forming heterocysts. The third, acclimation phase started first in the differentiating heterocysts with a recovery of PS II photochemical yields Afterwards, the onset of nitrogenase activity was observed, followed by the restoration of antenna pigments in the vegetative cells, but not in the heterocysts. Surprisingly, mature heterocysts were found to have an intact PS II as judged by photochemical yields, but a strongly reduced PS II-associated antenna as judged by decreased F (0). The possible importance of the functional PS II in heterocysts is discussed (en)
Title
  • Regulation of photosynthesis during heterocyst differentiation in Anabaena sp strain PCC 7120 investigated in vivo at single-cell level by chlorophyll fluorescence kinetic microscopy
  • Regulation of photosynthesis during heterocyst differentiation in Anabaena sp strain PCC 7120 investigated in vivo at single-cell level by chlorophyll fluorescence kinetic microscopy (en)
skos:prefLabel
  • Regulation of photosynthesis during heterocyst differentiation in Anabaena sp strain PCC 7120 investigated in vivo at single-cell level by chlorophyll fluorescence kinetic microscopy
  • Regulation of photosynthesis during heterocyst differentiation in Anabaena sp strain PCC 7120 investigated in vivo at single-cell level by chlorophyll fluorescence kinetic microscopy (en)
skos:notation
  • RIV/61388971:_____/13:00422189!RIV14-MSM-61388971
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(ED2.1.00/03.0110), P(GA206/08/1683), V
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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  • 102073
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/13:00422189
http://linked.open...riv/jazykVysledku
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  • Acclimation to abiotic stress; Heterocyst differentiation; Nitrogen fixation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [754C57B5720D]
http://linked.open...i/riv/nazevZdroje
  • Photosynthesis Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 116
http://linked.open...iv/tvurceVysledku
  • Prášil, Ondřej
  • Šedivá, Barbora
  • Šetlíková, Eva
  • Felcmanová, Kristina
  • Ferimazová, Naila
  • Hauska, G.
  • Küpper, H.
  • Maldener, I.
http://linked.open...ain/vavai/riv/wos
  • 000323666200007
issn
  • 0166-8595
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11120-013-9897-z
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