About: Vital mitochondrial functions show profound changes during yeast culture ageing

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About: Vital mitochondrial functions show profound changes during yeast culture ageing Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	During a 10-day culture ageing, cells of the wild-type Saccharomyces cerevisiae strain JC 482 retain their viability, while mitochondrial function and morphology change. Cell routine and uncoupled respiration rates increase to a maximum on day 4 and then decline to near zero. The decline, which occurs also in mitochondria isolated from cells of different age, is not due to increasing proportion of petites. Rhodamine 123 fluorescence intensity reporting on mitochondrial membrane potential appears to drop slightly for 4 days and then more sharply at the time when respiration rate also decreases. The MitoTracker Green fluorescent signal related to the mitochondrial content per cell also decreases. The branched tubular mitochondrial network of 1-day-old cells dissolves into short fragments; during the first 4 days, this fragmentation is associated with increasing function of mitochondria, while later on, it accompanies functional decline, which is also indicated by the decreasing ratio of Rhodamine 123 fluorescence to MitoTracker Green fluorescence During a 10-day culture ageing, cells of the wild-type Saccharomyces cerevisiae strain JC 482 retain their viability, while mitochondrial function and morphology change. Cell routine and uncoupled respiration rates increase to a maximum on day 4 and then decline to near zero. The decline, which occurs also in mitochondria isolated from cells of different age, is not due to increasing proportion of petites. Rhodamine 123 fluorescence intensity reporting on mitochondrial membrane potential appears to drop slightly for 4 days and then more sharply at the time when respiration rate also decreases. The MitoTracker Green fluorescent signal related to the mitochondrial content per cell also decreases. The branched tubular mitochondrial network of 1-day-old cells dissolves into short fragments; during the first 4 days, this fragmentation is associated with increasing function of mitochondria, while later on, it accompanies functional decline, which is also indicated by the decreasing ratio of Rhodamine 123 fluorescence to MitoTracker Green fluorescence (en)
Title	Vital mitochondrial functions show profound changes during yeast culture ageing Vital mitochondrial functions show profound changes during yeast culture ageing (en)
skos:prefLabel	Vital mitochondrial functions show profound changes during yeast culture ageing Vital mitochondrial functions show profound changes during yeast culture ageing (en)
skos:notation	RIV/61388971:_____/13:00396040!RIV14-MSM-61388971
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(1M0570), P(GA301/07/0339), P(ME09043)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hlousková, Jana Pichová, Alena Sigler, Karel Volejníková, Andrea
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	114227
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/13:00396040
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Saccharomyces cerevisiae; chronological ageing; mitochondria (en)
http://linked.open.../riv/klicoveSlovo	chronological ageing mitochondria Saccharomyces cerevisiae
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0B92905CB7CE]
http://linked.open...i/riv/nazevZdroje	FEMS Yeast Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Research Centre for Study of Extract Compounds of Barley and Hop Impact of mitochondrial status on aging and apoptosis in yeast: molecular and cell-biological approach Interplay between mitochondrial function and aging in yeast: molecular and cell-biological approach
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	13
http://linked.open...iv/tvurceVysledku	Hlousková, Jana Pichová, Alena Sigler, Karel Volejníková, Andrea
http://linked.open...ain/vavai/riv/wos	000313722000003
issn	1567-1356
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/1567-1364.12001

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