About: Corynebacterium glutamicum promoters: a practical approach

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Transcription initiation is the key step in gene expression in bacteria, and it is therefore studied for both theoretical and practical reasons. Promoters, the traffic lights of transcription initiation, are used as construction elements in biotechnological efforts to coordinate green waves' in the metabolic pathways leading to the desired metabolites. Detailed analyses of Corynebacterium glutamicum promoters have already provided large amounts of data on their structures, regulatory mechanisms and practical capabilities in metabolic engineering. In this minireview the main aspects of promoter studies, the methods developed for their analysis and their practical use in C.glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C.glutamicum promoter studies are outlined Transcription initiation is the key step in gene expression in bacteria, and it is therefore studied for both theoretical and practical reasons. Promoters, the traffic lights of transcription initiation, are used as construction elements in biotechnological efforts to coordinate green waves' in the metabolic pathways leading to the desired metabolites. Detailed analyses of Corynebacterium glutamicum promoters have already provided large amounts of data on their structures, regulatory mechanisms and practical capabilities in metabolic engineering. In this minireview the main aspects of promoter studies, the methods developed for their analysis and their practical use in C.glutamicum are discussed. These include definitions of the consensus sequences of the distinct promoter classes, promoter localization and characterization, activity measurements, the functions of transcriptional regulators and examples of practical uses of constitutive, inducible and modified promoters in biotechnology. The implications of the introduction of novel techniques, such as in vitro transcription and RNA sequencing, to C.glutamicum promoter studies are outlined (en)
Title	Corynebacterium glutamicum promoters: a practical approach Corynebacterium glutamicum promoters: a practical approach (en)
skos:prefLabel	Corynebacterium glutamicum promoters: a practical approach Corynebacterium glutamicum promoters: a practical approach (en)
skos:notation	RIV/61388971:_____/13:00396036!RIV14-AV0-61388971
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GPP302/12/P633)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Pátek, Miroslav Nešvera, Jan Holátko, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	67172
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/13:00396036
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	VITRO TRANSCRIPTION SYSTEM; L-LYSINE PRODUCTION; SIGMA-FACTOR SIGB (en)
http://linked.open.../riv/klicoveSlovo	L-LYSINE PRODUCTION SIGMA-FACTOR SIGB VITRO TRANSCRIPTION SYSTEM
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[20010A42E165]
http://linked.open...i/riv/nazevZdroje	Microbial Biotechnology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of in vitro transcription system for Corynebacterium glutamicum and characterization of RNA polymerase activity
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Nešvera, Jan Pátek, Miroslav Holátko, Jiří Busche, T. Kalinowski, J.
http://linked.open...ain/vavai/riv/wos	000315124400002
issn	1751-7907
number of pages	15 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/1751-7915.12019

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