About: Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping     Goto   Sponge   NotDistinct   Permalink

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  • Aims To perform a systematic evaluation of the applicability, validity and reliability of the long PCR-RFLP of 16S-ITS-23S rRNA genes for bacterial genotyping using both sequences retrieved from public genome databases and the experimental data obtained on bacterial cultures. Methods and Results 3301 Full-length sequences of 16S-ITS-23S rRNA genes were retrieved from 885 published bacterial genomes. Copy numbers of the whole set of 16S-ITS-23S rRNA genes per genome ranged from 1 (n = 161) to 14 (n = 4) with an average of 3.71. Their length varied greatly, from 4319 to 6568 bp with an average of 4952 bp. Computer-simulated RFLP analyses of the 16S-ITS-23S fragments flanked by the conserved primers 27F and 2241R suggested MspI, RsaI, HhaI and TaqI as the most appropriate enzymes for long PCR-RFLP analysis of the 16S-ITS-23S sequence
  • Aims To perform a systematic evaluation of the applicability, validity and reliability of the long PCR-RFLP of 16S-ITS-23S rRNA genes for bacterial genotyping using both sequences retrieved from public genome databases and the experimental data obtained on bacterial cultures. Methods and Results 3301 Full-length sequences of 16S-ITS-23S rRNA genes were retrieved from 885 published bacterial genomes. Copy numbers of the whole set of 16S-ITS-23S rRNA genes per genome ranged from 1 (n = 161) to 14 (n = 4) with an average of 3.71. Their length varied greatly, from 4319 to 6568 bp with an average of 4952 bp. Computer-simulated RFLP analyses of the 16S-ITS-23S fragments flanked by the conserved primers 27F and 2241R suggested MspI, RsaI, HhaI and TaqI as the most appropriate enzymes for long PCR-RFLP analysis of the 16S-ITS-23S sequence (en)
Title
  • Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping
  • Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping (en)
skos:prefLabel
  • Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping
  • Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping (en)
skos:notation
  • RIV/61388971:_____/13:00393101!RIV14-MSM-61388971
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  • I, P(ED2.1.00/03.0110)
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  • 2
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  • 85350
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  • RIV/61388971:_____/13:00393101
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  • 16S-ITS-23S; bacterial genome; computer simulation (en)
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  • GB - Spojené království Velké Británie a Severního Irska
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  • [9DC767472CD1]
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  • Journal of Applied Microbiology
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  • 114
http://linked.open...iv/tvurceVysledku
  • Koblížek, Michal
  • Zeng, Yonghui
  • Feng, F. Y.
  • Ji, J. D.
  • Jian, J. C.
  • Li, Y. X.
  • Liu, Y. P.
  • Wu, Z. H.
http://linked.open...ain/vavai/riv/wos
  • 000313723800015
issn
  • 1364-5072
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/jam.12057
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