About: DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress

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About: DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cytoplasmic membranes of B. subtilis were severely affected by even short-term exposure to 3% (v / v) ethanol: the growth rate and membrane protein synthesis were markedly reduced, and no adaptive alterations in phospholipids were detected. Simultaneously, steady-state DPH fluorescence anisotropy (rss) showed that the membrane rigidity increased substantially. Analysis of the membrane phosphoproteome using in vitro labelling with [c-32 P] ATP revealed the association of DnaK and GroEL chaperones with membrane, indicating a stress induction process. Upon a long-term 3% (v / v) ethanol stress, the cell growth accelerated slightly and the composition of polar head groups and fatty acids of membrane phospholipids underwent an extensive reconstruction. Correspondingly, membrane fluidity turned back to the original rss values of the control cells Cytoplasmic membranes of B. subtilis were severely affected by even short-term exposure to 3% (v / v) ethanol: the growth rate and membrane protein synthesis were markedly reduced, and no adaptive alterations in phospholipids were detected. Simultaneously, steady-state DPH fluorescence anisotropy (rss) showed that the membrane rigidity increased substantially. Analysis of the membrane phosphoproteome using in vitro labelling with [c-32 P] ATP revealed the association of DnaK and GroEL chaperones with membrane, indicating a stress induction process. Upon a long-term 3% (v / v) ethanol stress, the cell growth accelerated slightly and the composition of polar head groups and fatty acids of membrane phospholipids underwent an extensive reconstruction. Correspondingly, membrane fluidity turned back to the original rss values of the control cells (en)
Title	DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress (en)
skos:prefLabel	DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress DnaK and GroEL chaperones are recruited to the Bacillus subtilis membrane after short-term ethanol stress (en)
skos:notation	RIV/61388971:_____/12:00382747!RIV13-AV0-61388971
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(LC07017), S, Z(AV0Z50200510), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Halada, Petr Ulrych, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	131766
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/12:00382747
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bacillus subtilis; ethanol stress; membrane (en)
http://linked.open.../riv/klicoveSlovo	Bacillus subtilis membrane ethanol stress
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[965024695715]
http://linked.open...i/riv/nazevZdroje	Journal of Applied Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Oncoproteomics Centre
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	112
http://linked.open...iv/tvurceVysledku	Halada, Petr Svobodová, J. Ulrych, Aleš Toman, O. Fišer, R. Seydlová, G.
http://linked.open...ain/vavai/riv/wos	000301432200015
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Signaling and cellular response mechanisms
issn	1364-5072
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1365-2672.2012.05238.x

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