About: Long-term tracing of Rhizophagus irregularis isolate BEG140 inoculated on Phalaris arundinacea in a coal mine spoil bank, using mitochondrial large subunit rDNA markers

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About: Long-term tracing of Rhizophagus irregularis isolate BEG140 inoculated on Phalaris arundinacea in a coal mine spoil bank, using mitochondrial large subunit rDNA markers Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Using mitochondrial large subunit ribosomal DNA, we show that the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis isolate BEG140 consists of two different haplotypes. We developed nested PCR assays to specifically trace each of the two haplotypes in the roots of Phalaris arundinacea from a field experiment in a spoil bank of a former coal mine, where BEG140 was used as inoculant. We revealed that despite the relatively high diversity of native R. irregularis strains, R. irregularis BEG140 survived and proliferated successfully in the field experiment and was found significantly more often in the inoculated than control plots. This work is the first one to show tracing of an inoculated AMF isolate in the roots of target plants and to verify its survival and propagation in the field. Using mitochondrial large subunit ribosomal DNA, we show that the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis isolate BEG140 consists of two different haplotypes. We developed nested PCR assays to specifically trace each of the two haplotypes in the roots of Phalaris arundinacea from a field experiment in a spoil bank of a former coal mine, where BEG140 was used as inoculant. We revealed that despite the relatively high diversity of native R. irregularis strains, R. irregularis BEG140 survived and proliferated successfully in the field experiment and was found significantly more often in the inoculated than control plots. This work is the first one to show tracing of an inoculated AMF isolate in the roots of target plants and to verify its survival and propagation in the field. (en)
Title	Long-term tracing of Rhizophagus irregularis isolate BEG140 inoculated on Phalaris arundinacea in a coal mine spoil bank, using mitochondrial large subunit rDNA markers Long-term tracing of Rhizophagus irregularis isolate BEG140 inoculated on Phalaris arundinacea in a coal mine spoil bank, using mitochondrial large subunit rDNA markers (en)
skos:prefLabel	Long-term tracing of Rhizophagus irregularis isolate BEG140 inoculated on Phalaris arundinacea in a coal mine spoil bank, using mitochondrial large subunit rDNA markers Long-term tracing of Rhizophagus irregularis isolate BEG140 inoculated on Phalaris arundinacea in a coal mine spoil bank, using mitochondrial large subunit rDNA markers (en)
skos:notation	RIV/61388971:_____/12:00374124!RIV12-AV0-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0571), P(GPP504/10/P021), Z(AV0Z50200510), Z(AV0Z60050516)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Gryndler, Milan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	147446
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/12:00374124
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Rhizophagus irregularis; inoculation; isolate tracing (en)
http://linked.open.../riv/klicoveSlovo	inoculation Rhizophagus irregularis isolate tracing
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[9EE7B457AA2B]
http://linked.open...i/riv/nazevZdroje	Mycorrhiza
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Bioindication and revitalization of toxic and antropogenic substrates and water sources: application of cyano bacteria, algae, soil bacteria and symbiotic fungi Tracing of arbuscular mycorrhizal fungi (AMF) isolates inoculated into the field and evaluation of subsequent AMF community structure changes
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	22
http://linked.open...iv/tvurceVysledku	Sýkorová, Zuzana Gryndler, Milan Vosátka, Miroslav Fehrer, Judith Redecker, D. Börstler, B. Zvolenská, Soňa
http://linked.open...ain/vavai/riv/wos	000299327000006
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Struktura, funkce a evoluce biodiversity fotoautotrofních organismů a hub: původ a příčiny jejich proměnlivosti, dynamika populací, společenstev a ekosystémů; využití vybraných výsledků k rozvoji Průhonického parku
issn	0940-6360
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00572-011-0375-1

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