About: 5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two novel 5D NMR experiments (CACONCACO, NCOCANCO) for backbone assignment of disordered proteins are presented. The pulse sequences exploit relaxation properties of the unstructured proteins and combine the advantages of 13C-direct detection, non-uniform sampling, and longitudinal relaxation optimization to maximize the achievable resolution and minimize the experimental time. The pulse sequences were successfully tested on the sample of partially disordered delta subunit from RNA polymerase from Bacillus subtilis. The unstructured part of this 20 kDa protein consists of 81 amino acids with frequent sequential repeats. A collection of 0.0003% of the data needed for a conventional experiment with linear sampling was sufficient to perform an unambiguous assignment of the disordered part of the protein from a single 5D spectrum Two novel 5D NMR experiments (CACONCACO, NCOCANCO) for backbone assignment of disordered proteins are presented. The pulse sequences exploit relaxation properties of the unstructured proteins and combine the advantages of 13C-direct detection, non-uniform sampling, and longitudinal relaxation optimization to maximize the achievable resolution and minimize the experimental time. The pulse sequences were successfully tested on the sample of partially disordered delta subunit from RNA polymerase from Bacillus subtilis. The unstructured part of this 20 kDa protein consists of 81 amino acids with frequent sequential repeats. A collection of 0.0003% of the data needed for a conventional experiment with linear sampling was sufficient to perform an unambiguous assignment of the disordered part of the protein from a single 5D spectrum (en)
Title	5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion 5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion (en)
skos:prefLabel	5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion 5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion (en)
skos:notation	RIV/61388971:_____/11:00367828!RIV12-AV0-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/09/0583), P(GAP206/11/0758), P(GD301/09/H004), P(LC06030), Z(AV0Z50200510), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Krásný, Libor Šanderová, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	244201
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/11:00367828
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Intrinsically disordered proteins; Non-uniform sampling; Longitudinal relaxation optimization (en)
http://linked.open.../riv/klicoveSlovo	Intrinsically disordered proteins Longitudinal relaxation optimization Non-uniform sampling
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C74C22C77EA3]
http://linked.open...i/riv/nazevZdroje	Journal of Biomolecular NMR
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	The Delta Subunit of RNA Polymerase from Gram positive Bacteria Development of high-resolution methodology for NMR studies of disordered proteins with highly degenerated resonance frequencies Molecular and structural biology of selected antitumor drugs. From mechanistic studies to chemotherapy of tumors Biomolecular centre
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Krásný, Libor Šanderová, Hana Sklenář, V. Kozminski, W. Nováček, J. Papoušková, V. Zawadzka-Kazimierczuk, A. Žídek, L.
http://linked.open...ain/vavai/riv/wos	000290044400001
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Proteiny v metabolismu a při interakci organismů s prostředím
issn	0925-2738
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10858-011-9496-2

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