About: A new approach to the isolation and characterization of wheat flour allergens

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The incidence of food allergy to wheat is increasing. Its diagnosis depends on the purity of major allergens and their inclusion in tests. Isolation and characterization of wheat allergens are therefore of utmost importance. Objective To purify and identify wheat flour allergens most frequently recognized by patients IgE antibodies and to study their allergenicity. Methods Water/salt-soluble extracts from wheat flour were prepared and separated using a combination of ultrafiltration, isoelectric focusing and liquid chromatography. Purified proteins were analysed by immunoblotting using pooled sera from patients with atopic dermatitis who possessed IgE specific to wheat. The incidence of food allergy to wheat is increasing. Its diagnosis depends on the purity of major allergens and their inclusion in tests. Isolation and characterization of wheat allergens are therefore of utmost importance. Objective To purify and identify wheat flour allergens most frequently recognized by patients IgE antibodies and to study their allergenicity. Methods Water/salt-soluble extracts from wheat flour were prepared and separated using a combination of ultrafiltration, isoelectric focusing and liquid chromatography. Purified proteins were analysed by immunoblotting using pooled sera from patients with atopic dermatitis who possessed IgE specific to wheat. (en)
Title	A new approach to the isolation and characterization of wheat flour allergens A new approach to the isolation and characterization of wheat flour allergens (en)
skos:prefLabel	A new approach to the isolation and characterization of wheat flour allergens A new approach to the isolation and characterization of wheat flour allergens (en)
skos:notation	RIV/61388971:_____/11:00362802!RIV12-AV0-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06155), P(GA310/07/0414), P(GD310/08/H077), P(IAA500200801), P(LC07017), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Halada, Petr Kainarová, Andrea Šotkovský, Petr Tučková, Ludmila Cinová, Jana Sklenář, Jan Goliáš, Jaroslav Pavlásková, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	183931
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/11:00362802
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	basophil activation; IgE antibodies; isolation (en)
http://linked.open.../riv/klicoveSlovo	isolation IgE antibodies basophil activation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[896C1A23BA4F]
http://linked.open...i/riv/nazevZdroje	Clinical and Experimental Allergy
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Identification of new markers for molecular diagnosis of inflammatory and tumor diseases. The role of innate and adaptive immunity in development of gluten-sensitive entheropathy Regulation of immunological mechanisms in health and disease:Development of new diagnostic and therapeutic approaches Food allergy to wheat proteins Oncoproteomics Centre
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	41
http://linked.open...iv/tvurceVysledku	Cinová, Jana Goliáš, Jaroslav Halada, Petr Honzová, S. Kainarová, Andrea Pavlásková, Kateřina Sklenář, Jan Tučková, Ludmila Šetinová, I. Šotkovský, Petr
http://linked.open...ain/vavai/riv/wos	000292699700016
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0954-7894
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1365-2222.2011.03766.x

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