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  • Desorption electrospray ionization (DESI) mass spectrometry appears to be a useful technique applicable in different areas (e.g. analysis of pharmaceuticals, identification of biologically active compounds in tissues, imaging mass spectrometry). Its modification termed desorption nano-electrospray (nano-DESI) was tested for analysis of anthocyanins. Acidifying of samples and acidic spray liquid (methanol:water = 75:25 with 0.2% HCOOH) were essential for obtaining good quality spectra. Profiles of main anthocyanins in wine samples, two vintages (2005 and 2007) of three cultivars (Alibernet, Neronet and Rubinet), were successfully acquired. They were in agreement with results of LC/MS experiments (anthocyanins isolated by solid phase extraction were separated by mu-HPLC with gradient elution and detected by ESI-MS). Nano-DESI-MS data also allowed to determine ratio of two cultivars (Neronet and Rubinet) in their mixture and to detect coloring of wine by tenturier or elderberry extract. Detection of main anthocyanins in slices of wine grape, chokeberries and elderberries or in a wine stain on cotton fabric is also presented.
  • Desorption electrospray ionization (DESI) mass spectrometry appears to be a useful technique applicable in different areas (e.g. analysis of pharmaceuticals, identification of biologically active compounds in tissues, imaging mass spectrometry). Its modification termed desorption nano-electrospray (nano-DESI) was tested for analysis of anthocyanins. Acidifying of samples and acidic spray liquid (methanol:water = 75:25 with 0.2% HCOOH) were essential for obtaining good quality spectra. Profiles of main anthocyanins in wine samples, two vintages (2005 and 2007) of three cultivars (Alibernet, Neronet and Rubinet), were successfully acquired. They were in agreement with results of LC/MS experiments (anthocyanins isolated by solid phase extraction were separated by mu-HPLC with gradient elution and detected by ESI-MS). Nano-DESI-MS data also allowed to determine ratio of two cultivars (Neronet and Rubinet) in their mixture and to detect coloring of wine by tenturier or elderberry extract. Detection of main anthocyanins in slices of wine grape, chokeberries and elderberries or in a wine stain on cotton fabric is also presented. (en)
Title
  • Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry
  • Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry (en)
skos:prefLabel
  • Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry
  • Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry (en)
skos:notation
  • RIV/61388971:_____/10:00434994!RIV15-GA0-61388971
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/07/0765), Z(AV0Z50200510), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 25
http://linked.open...vai/riv/dodaniDat
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  • 258836
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/10:00434994
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  • Mass spectrometry; Desorption nano-electrospray; Liquid chromatography (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [F7289779F6ED]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography A
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http://linked.open...v/svazekPeriodika
  • 1217
http://linked.open...iv/tvurceVysledku
  • Lemr, Karel
  • Havlíček, Vladimír
  • Bednář, P.
  • Papoušková, B.
  • Hartmanová, L.
  • Ranc, V.
http://linked.open...ain/vavai/riv/wos
  • 000278779000031
http://linked.open...n/vavai/riv/zamer
issn
  • 0021-9673
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.chroma.2010.03.018
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