About: Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry

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About: Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The intraocular lens contains high levels of both cholesterol and sphingolipids, which are believed to be functionally important for normal lens physiology. The aim of this study was to explore the spatial distribution of sphingolipids in the ocular lens using mass spectrometry imaging (MSI). Matrix-assisted laser desorption/ionization (MALDI) imaging with ultra high resolution Fourier transform ion cyklotron resonance mass spectrometry (FT-ICR MS) was used to visualize the lipid spatial distribution. Equatorially-cryosectioned, 12 um thick slices of tissue were thaw-mounted to an indium-tin oxide (ITO) glass slide by soft-landing to an ethanol layer. After the automated MALDI matrix deposition, the entire lens section was examined by MALDI MSI in a 150 um raster. We obtained spatial and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers The intraocular lens contains high levels of both cholesterol and sphingolipids, which are believed to be functionally important for normal lens physiology. The aim of this study was to explore the spatial distribution of sphingolipids in the ocular lens using mass spectrometry imaging (MSI). Matrix-assisted laser desorption/ionization (MALDI) imaging with ultra high resolution Fourier transform ion cyklotron resonance mass spectrometry (FT-ICR MS) was used to visualize the lipid spatial distribution. Equatorially-cryosectioned, 12 um thick slices of tissue were thaw-mounted to an indium-tin oxide (ITO) glass slide by soft-landing to an ethanol layer. After the automated MALDI matrix deposition, the entire lens section was examined by MALDI MSI in a 150 um raster. We obtained spatial and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers (en)
Title	Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry (en)
skos:prefLabel	Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry (en)
skos:notation	RIV/61388971:_____/10:00354289!RIV11-MSM-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC07017), Z(AV0Z50200510), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Novák, Petr Volný, Michael Pól, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	295855
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/10:00354289
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ocular lens; sphingolipid; matrix-assisted laser desorption/ionization (en)
http://linked.open.../riv/klicoveSlovo	sphingolipid matrix-assisted laser desorption/ionization ocular lens
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[BC179D09593F]
http://linked.open...i/riv/nazevZdroje	Journal of Lipid Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Oncoproteomics Centre
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Novák, Petr Volný, Michael Holopainen, J. Pól, Jaroslav Wiedmer, S. K. Vidová, V.
http://linked.open...ain/vavai/riv/wos	000279896800025
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0022-2275
number of pages	8 (xsd:int)

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