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rdf:type
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Description
| - The intraocular lens contains high levels of both cholesterol and sphingolipids, which are believed to be functionally important for normal lens physiology. The aim of this study was to explore the spatial distribution of sphingolipids in the ocular lens using mass spectrometry imaging (MSI). Matrix-assisted laser desorption/ionization (MALDI) imaging with ultra high resolution Fourier transform ion cyklotron resonance mass spectrometry (FT-ICR MS) was used to visualize the lipid spatial distribution. Equatorially-cryosectioned, 12 um thick slices of tissue were thaw-mounted to an indium-tin oxide (ITO) glass slide by soft-landing to an ethanol layer. After the automated MALDI matrix deposition, the entire lens section was examined by MALDI MSI in a 150 um raster. We obtained spatial and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers
- The intraocular lens contains high levels of both cholesterol and sphingolipids, which are believed to be functionally important for normal lens physiology. The aim of this study was to explore the spatial distribution of sphingolipids in the ocular lens using mass spectrometry imaging (MSI). Matrix-assisted laser desorption/ionization (MALDI) imaging with ultra high resolution Fourier transform ion cyklotron resonance mass spectrometry (FT-ICR MS) was used to visualize the lipid spatial distribution. Equatorially-cryosectioned, 12 um thick slices of tissue were thaw-mounted to an indium-tin oxide (ITO) glass slide by soft-landing to an ethanol layer. After the automated MALDI matrix deposition, the entire lens section was examined by MALDI MSI in a 150 um raster. We obtained spatial and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers (en)
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Title
| - Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry
- Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry (en)
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skos:prefLabel
| - Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry
- Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry (en)
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skos:notation
| - RIV/61388971:_____/10:00354289!RIV11-MSM-61388971
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(LC07017), Z(AV0Z50200510), Z(MSM6198959216)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/10:00354289
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - ocular lens; sphingolipid; matrix-assisted laser desorption/ionization (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Lipid Research
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Novák, Petr
- Volný, Michael
- Holopainen, J.
- Pól, Jaroslav
- Wiedmer, S. K.
- Vidová, V.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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