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  • Lilac coloured species of Geosmithia lavendula produce a mixture of polyhydroxylated anthraquinones under condition of submerged fermentation. Three pigments had been isolated and identified earlier as a 1,3,6,8-tetrahydroxyanthraquinone (compound 7), rhodolamprometrin (1-acetyl 2,4,5,7-tetrahydroxyanthraquinone; compound 5), and 1-acetyl 2,4,5,7,8-penthahydroxyanthraquinone (compound 4). A new HPLC method was developed for the separation of three known and ten new anthraquinone pigments. In addition, five new pigments were determined by FTMS as coeluting impurities. The analyses were performed on a reversed phase column using gradient elution with a mobile phase system consisting of phosphate buffer (50mM, pH 2.0) and acetonitrile. The structure evaluation was based namely on FTMS and UV–VIS spectrometry. The developed procedure was used for the determination of individual anthraquinones in fermentation broth of G. lavendula after 14 days of cultivation
  • Lilac coloured species of Geosmithia lavendula produce a mixture of polyhydroxylated anthraquinones under condition of submerged fermentation. Three pigments had been isolated and identified earlier as a 1,3,6,8-tetrahydroxyanthraquinone (compound 7), rhodolamprometrin (1-acetyl 2,4,5,7-tetrahydroxyanthraquinone; compound 5), and 1-acetyl 2,4,5,7,8-penthahydroxyanthraquinone (compound 4). A new HPLC method was developed for the separation of three known and ten new anthraquinone pigments. In addition, five new pigments were determined by FTMS as coeluting impurities. The analyses were performed on a reversed phase column using gradient elution with a mobile phase system consisting of phosphate buffer (50mM, pH 2.0) and acetonitrile. The structure evaluation was based namely on FTMS and UV–VIS spectrometry. The developed procedure was used for the determination of individual anthraquinones in fermentation broth of G. lavendula after 14 days of cultivation (en)
Title
  • High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula
  • High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula (en)
skos:prefLabel
  • High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula
  • High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula (en)
skos:notation
  • RIV/61388971:_____/10:00351441!RIV11-MSM-61388971
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B08064), P(KAN200200651), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika
  • 40
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 261589
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/10:00351441
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • High-performance liquid chromatography; Mass spectrometry; Anthraquinones (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [FC142E733708]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography A
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1217
http://linked.open...iv/tvurceVysledku
  • Flieger, Miroslav
  • Man, Petr
  • Kolařík, Miroslav
  • Stodůlková, Eva
http://linked.open...ain/vavai/riv/wos
  • 000282725800020
http://linked.open...n/vavai/riv/zamer
issn
  • 0021-9673
number of pages
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