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rdf:type
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Description
| - Paper presents a complete sample handling protocol, which allows processing of disulfide containing proteins at basic pH. We modified the standard SDS gel electrophoresis and protein digestion conditions by the addition of an oxidative agent, cystamine. Thismodification prevented disulfide scrambling, which we otherwise observed in the samples handled according to the general protocol. Lysozyme from hen egg was used as a model protein for the development of the method. Disulfide bonds were characterized in the following proteins - human leukocyte antigen CD69, murine leukocyte receptor NKR-P1A and glycosylated β-N-acetylhexosaminidases from Aspergillus oryzae and Penicillium oxalicum
- Paper presents a complete sample handling protocol, which allows processing of disulfide containing proteins at basic pH. We modified the standard SDS gel electrophoresis and protein digestion conditions by the addition of an oxidative agent, cystamine. Thismodification prevented disulfide scrambling, which we otherwise observed in the samples handled according to the general protocol. Lysozyme from hen egg was used as a model protein for the development of the method. Disulfide bonds were characterized in the following proteins - human leukocyte antigen CD69, murine leukocyte receptor NKR-P1A and glycosylated β-N-acetylhexosaminidases from Aspergillus oryzae and Penicillium oxalicum (en)
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Title
| - Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds
- Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds (en)
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skos:prefLabel
| - Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds
- Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds (en)
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skos:notation
| - RIV/61388971:_____/09:00337531!RIV10-MSM-61388971
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(IAA5020403), P(KJB400200501), P(KJB500200612), P(LC07017), P(LC545), Z(AV0Z50200510), Z(MSM0021620808), Z(MSM0021620835), Z(MSM6198959216)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/09:00337531
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - disulfide bond; cystamine; gel electrophoresis (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Mass Spectrometry
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bezouška, Karel
- Hofbauerová, Kateřina
- Kavan, Daniel
- Man, Petr
- Novák, Petr
- Havlíček, Vladimír
- Pompach, Petr
- Kumar, Vinay
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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