About: Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation

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About: Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The topic of this paper was modulation of expression of the genes involved in the biosynthesis of branched-chain amino acids in Corynebacterium glutamicum. The activity of the promoters P-ilvD (dihydroxyacid dehydratase) and P-ilvE (transaminase B) was up-modulated and the activity of the promoters P-ilvA (threonine deaminase) and P-leuA (isopropylmalate synthase) was down-modulated by site-directed mutagenesis. The previously constructed strain C. glutamicum ilvNM13 with acetohydroxy acid synthase, resistant to inhibition by all three branched-chain amino acids (L-valine, L-isoleucine and L-leucine), was used as a basis to develop a new type of valine producer by genetic engineering. A constructed weak promoter of ilvA (or leuA), which was introduced into the C. glutamicum chromosome via a gene-replacement technique reduced the biosynthetic rate of isoleucine (or leucine), which lowered the mutant growth rate and increased valine production The topic of this paper was modulation of expression of the genes involved in the biosynthesis of branched-chain amino acids in Corynebacterium glutamicum. The activity of the promoters P-ilvD (dihydroxyacid dehydratase) and P-ilvE (transaminase B) was up-modulated and the activity of the promoters P-ilvA (threonine deaminase) and P-leuA (isopropylmalate synthase) was down-modulated by site-directed mutagenesis. The previously constructed strain C. glutamicum ilvNM13 with acetohydroxy acid synthase, resistant to inhibition by all three branched-chain amino acids (L-valine, L-isoleucine and L-leucine), was used as a basis to develop a new type of valine producer by genetic engineering. A constructed weak promoter of ilvA (or leuA), which was introduced into the C. glutamicum chromosome via a gene-replacement technique reduced the biosynthetic rate of isoleucine (or leucine), which lowered the mutant growth rate and increased valine production (en)
Title	Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation (en)
skos:prefLabel	Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation (en)
skos:notation	RIV/61388971:_____/09:00327369!RIV10-AV0-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/06/0330), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Prouza, Marek Pátek, Miroslav Nešvera, Jan Holátko, Jiří Sobotka, Miroslav Elišáková, Veronika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	325563
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/09:00327369
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	corynebacterium glutamicum; valine production; promoters (en)
http://linked.open.../riv/klicoveSlovo	promoters corynebacterium glutamicum valine production
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[23803AB73946]
http://linked.open...i/riv/nazevZdroje	Journal of Biotechnology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Expression of Corynebacterium glutamicum genes in the stationary phase of growth
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	139
http://linked.open...iv/tvurceVysledku	Prouza, Marek Nešvera, Jan Pátek, Miroslav Holátko, Jiří Sobotka, Miroslav Elišáková, Veronika
http://linked.open...ain/vavai/riv/wos	000263742300002
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0168-1656
number of pages	8 (xsd:int)

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