About: Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase

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About: Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase – A way to new glycodrugs and materials Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Human placental b1,4-galactosyltransferase-I (EC 2.4.1.38) transfers the galactosyl moiety from UDP-Gal to various GlcNAc or Glc acceptors in vivo. Here, we describe the construction of its Y284L mutant as a His6propeptidecatb4GalT1 construct, in which the Gal-transferase aktivity was totally abolished in favor of its GalNAc-transferase activity. We used this mutant in the synthesis of three monoand bivalent LacdiNAc glycomimetics with good yields. These compounds proved to be powerful ligands of two activation receptors of natural killer cells, NKR-P1 and CD69.A synthetic bivalent tethered di-LacdiNAc is the best currently known precipitation agent for both of these receptors and has promising potential for the development of immunoactive glycodrugs Human placental b1,4-galactosyltransferase-I (EC 2.4.1.38) transfers the galactosyl moiety from UDP-Gal to various GlcNAc or Glc acceptors in vivo. Here, we describe the construction of its Y284L mutant as a His6propeptidecatb4GalT1 construct, in which the Gal-transferase aktivity was totally abolished in favor of its GalNAc-transferase activity. We used this mutant in the synthesis of three monoand bivalent LacdiNAc glycomimetics with good yields. These compounds proved to be powerful ligands of two activation receptors of natural killer cells, NKR-P1 and CD69.A synthetic bivalent tethered di-LacdiNAc is the best currently known precipitation agent for both of these receptors and has promising potential for the development of immunoactive glycodrugs (en)
Title	Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase – A way to new glycodrugs and materials Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase – A way to new glycodrugs and materials (en)
skos:prefLabel	Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase – A way to new glycodrugs and materials Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase – A way to new glycodrugs and materials (en)
skos:notation	RIV/61388971:_____/09:00327139!RIV10-MSM-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/06/0771), P(GP203/09/P024), P(LC06010), P(OC 136), Z(AV0Z50200510), Z(MSM0021620808)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bezouška, Karel Bojarová, Pavla Křen, Vladimír Křenek, Karel Pelantová, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	345110
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/09:00327139
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	enzymatic synthesis; glycomimetics; glycosyltransferase (en)
http://linked.open.../riv/klicoveSlovo	glycosyltransferase enzymatic synthesis glycomimetics
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[5B681064A7F0]
http://linked.open...i/riv/nazevZdroje	Glycobiology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Biocatalysis and Biotransformation http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20136 Synthesis of immunoactive glycosides by beta-N-acetylhexosaminidases The use of surface glycosylation in the immune recognition and elimination of tumors
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Bezouška, Karel Bojarová, Pavla Elling, L. Křen, Vladimír Křenek, Karel Pelantová, Helena Adamiak, K. Wetjen, K.
http://linked.open...ain/vavai/riv/wos	000265096000007
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu
issn	0959-6658
number of pages	9 (xsd:int)

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