About: Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was used to activate the carboxylic acid containing residues, aspartic acid (D), glutamic acid (E), and the carboxy terminus (O), for cross-linking reactions. Once activated, the DEO sidechains can react to form “zero-length cross-links with nearby primary amine containing residues, lysines (K) and the amino terminus (X), via the formation of a new amide bond. Also the EDC-activated DEO sidechains can be cross-linked to each other using dihydrazides, two hydrazide moieties connected by an alkyl cross-linker arm of variable length 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was used to activate the carboxylic acid containing residues, aspartic acid (D), glutamic acid (E), and the carboxy terminus (O), for cross-linking reactions. Once activated, the DEO sidechains can react to form “zero-length cross-links with nearby primary amine containing residues, lysines (K) and the amino terminus (X), via the formation of a new amide bond. Also the EDC-activated DEO sidechains can be cross-linked to each other using dihydrazides, two hydrazide moieties connected by an alkyl cross-linker arm of variable length (en) 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) byl použit k aktivaci postranních karboxylových skupin aminokyselin (kyseliny glutamová, asparagová a C-konec) pro zesíťovácí reakci. Jakmile je karboxylový zbytek aktivovaný tvoří „nulové“ zesítění (lidická vazba) s blízkou aminoskupinou (lysin a N konec). Takto aktivovanékarboxylové zbytky také můžou v přítomnosti dihydrazidů o různých velikostech tvořit zesítění mezi sebou (cs)
Title	Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies Využití intramolekulárního zesítění postranních karboxylových skupin pro studium struktury proteinů.. (cs) Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies (en)
skos:prefLabel	Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies Využití intramolekulárního zesítění postranních karboxylových skupin pro studium struktury proteinů.. (cs) Intra-molecular Cross-linking of Acidic Residues for Protein Structure Studies (en)
skos:notation	RIV/61388971:_____/08:00320965!RIV09-AV0-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC545), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Novák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	373080
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/08:00320965
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	proteins; top-down; ft-ms (en)
http://linked.open.../riv/klicoveSlovo	proteins ft-ms top-down
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[EDB224D26982]
http://linked.open...i/riv/nazevZdroje	European Journal of Mass Spectrometry
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional Organisation of the Cell
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Novák, Petr Kruppa, G. H.
http://linked.open...ain/vavai/riv/wos	000262813100004
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	1469-0667
number of pages	11 (xsd:int)

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