About: SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation

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About: SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byly získány nové poznatky o struktuře tmRNA průmyslově významných producentů antibiotik S. aureofaciens, S. griseus a S. coelicolor. 2) Byla objasněna asociaci proteinů s tmRNA v průběhu růstu S. aureofaciens. 3) Byla vysvětlena funkce ribosomálního proteinu SS1 při trans-translaci. 4) Získali jsme původní informace o struktuře proteinu SmpB u S. aureofaciens a jeho hladinách u buněk z exponenciální a stacionární fáze růstu (cs) Comparative sequence analysis of ssrA genes of streptomycetes revealed the most significant differences in the central parts of tag-reading frames, in the stop codons and in the 15-34 nucleotide sequences following stop codons. Proteins from various phases of development of Streptomyces aureofaciens associated with tmRNA were analyzed. Using affinity chromatography on tmRNA-Sepharose and photo cross-linking experiments with [32P]labelled tmRNA seven proteins, were identified among the proteins associated with tmRNA of Streptomyces aureofaciens. We examined functional role of ribosomal protein SS1 in defined in vitro trans-translation system. Our data show that the protein SS1 that structurally differ from S1 of Escherichia coli is required for translation of the tmRNA tag-reading frame Comparative sequence analysis of ssrA genes of streptomycetes revealed the most significant differences in the central parts of tag-reading frames, in the stop codons and in the 15-34 nucleotide sequences following stop codons. Proteins from various phases of development of Streptomyces aureofaciens associated with tmRNA were analyzed. Using affinity chromatography on tmRNA-Sepharose and photo cross-linking experiments with [32P]labelled tmRNA seven proteins, were identified among the proteins associated with tmRNA of Streptomyces aureofaciens. We examined functional role of ribosomal protein SS1 in defined in vitro trans-translation system. Our data show that the protein SS1 that structurally differ from S1 of Escherichia coli is required for translation of the tmRNA tag-reading frame (en)
Title	SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation (en) SsrA geny streptomycet a asociace proteinů s tmRNA během vývoje a buněčné diferenciace (cs)
skos:prefLabel	SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation SsrA genes of streptomycetes and association of proteins to the tmRNA during developm ent and cellular differentiation (en) SsrA geny streptomycet a asociace proteinů s tmRNA během vývoje a buněčné diferenciace (cs)
skos:notation	RIV/61388971:_____/08:00313096!RIV09-AV0-61388971
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA203/05/0106), S, Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Halada, Petr Felsberg, Jürgen Feglarová, Petra Mikulík, Karel Bobek, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	396831
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/08:00313096
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	in vitro trans-translation; ribosomal protein ss1; streptomycetes (en)
http://linked.open.../riv/klicoveSlovo	streptomycetes in vitro trans-translation ribosomal protein ss1
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[82A3CD5DBC82]
http://linked.open...i/riv/nazevZdroje	Proteomics
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of trans-translation in cellular differentiation and resistance to produced antibiotics in Streptomycetes
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Halada, Petr Felsberg, Jürgen Bobek, Jan Mikulík, Karel Zídková, J. Palečková, Petra
http://linked.open...ain/vavai/riv/wos	000254986200008
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	1615-9853
number of pages	13 (xsd:int)

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