| Attributes | Values |
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| rdf:type
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| Description
| - Prezentovaný článek popisuje stanovení aktivity tyroxin-hydroxylázy,která je jedním z enzymů v biosyntetické dráze linkomycinu. Metoda obsahuje vývoj HPLC metody, s isokratickou elucí a a fluorescenční detekcí. Při validaci této metody byl určen lineární rozsah pro DOPU 0.125–25 mmol/L s nejnižším limitem detekce LLOQ 0.125 mmol/L, RSD 7.2 percent a přesností 101.7 percent mmol/L a lineární rozsah pro tyroxin 15.625 mmol/L až 500 mmol/L s LLOQ 15.625 mmol/L, RSD 1.1 percent, a přesností 98.1 percent. Byla stanovena výtěžnost pro Dopa 100.66 percent s RSD 61472;0.89 percent a pro Tyr 94.76 percent s RSD 61472;0.94 percent. Byla stanovena přesnost a správnost metody v testu inter- and intra-day assay, která nepřekročila 10 procent. Dále byla určena stabilita vzorků reakční směsi při pokojové a 8722;20 stupňů C teplotě. Pomocí této metody bylo optimalizováno složení směsi enzymatické reakce a dále byly zjištěny podmínky pro zabránění vzdušné (neenzymatické) oxidace (cs)
- An assay of L-tyrosine (Tyr) hydroxylating activity operating in lincomycin biosynthesis is described. The assay development consisted of HPLC procedure development, assessing the effect of reaction mixture components on non-enzymatic. Dopa and Tyr oxidation, and sample stability evaluation. The HPLC procedure with isocratic elution and fluorescence detection was developed and validated. The method showed a wide linear range of Dopa determination of 0.125–25 mmol/L with lower limit of quantification (LLOQ) of 0.125 mmol/L, RSD of 7.2 percent and accuracy of 101.7 percent. The studied linear range of Tyr was 15.625 mmol/L to 500 mmol/L with LLOQ of 15.625 mmol/L, RSD of 1.1percent, and accuracy of 98.1percent. Recoveries for Dopa and Tyr were 100.66 percent RSD 61472;0.89 percent and 94.76 percent RSD 61472;0.94 percent, respectively
- An assay of L-tyrosine (Tyr) hydroxylating activity operating in lincomycin biosynthesis is described. The assay development consisted of HPLC procedure development, assessing the effect of reaction mixture components on non-enzymatic. Dopa and Tyr oxidation, and sample stability evaluation. The HPLC procedure with isocratic elution and fluorescence detection was developed and validated. The method showed a wide linear range of Dopa determination of 0.125–25 mmol/L with lower limit of quantification (LLOQ) of 0.125 mmol/L, RSD of 7.2 percent and accuracy of 101.7 percent. The studied linear range of Tyr was 15.625 mmol/L to 500 mmol/L with LLOQ of 15.625 mmol/L, RSD of 1.1percent, and accuracy of 98.1percent. Recoveries for Dopa and Tyr were 100.66 percent RSD 61472;0.89 percent and 94.76 percent RSD 61472;0.94 percent, respectively (en)
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| Title
| - Assay of tyrosine hydroxylase based on high-performance
- Assay of tyrosine hydroxylase based on high-performance (en)
- Metoda stanovení tyrosin-hydroxylázy založená na HPLC separaci a stanovení L-dopy a L-tyrosinu (cs)
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| skos:prefLabel
| - Assay of tyrosine hydroxylase based on high-performance
- Assay of tyrosine hydroxylase based on high-performance (en)
- Metoda stanovení tyrosin-hydroxylázy založená na HPLC separaci a stanovení L-dopy a L-tyrosinu (cs)
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| skos:notation
| - RIV/61388971:_____/07:00092260!RIV08-AV0-61388971
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1M06011), P(GA204/04/0801), P(GA204/05/0616), Z(AV0Z50200510)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/07:00092260
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - hplc with fluorescence detection; dopa and tyrosine determination; tyrosine hydroxylating activity assay (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Biomedical Chromatography
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Flieger, Miroslav
- Olšovská, Jana
- Novotná, Jitka
- Spížek, Jaroslav
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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