About: Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus

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About: Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	An extracellular alfa-D-galactosidase from Talaromyces flavus CCF 2686 with extremely broad and unusual acceptor specificity is produced exclusively in the presence of the specific inducer - 6-deoxy-D-glucose (quinovose). The procedure for the preparation of this very expensive substance has been modified and optimized. Surprisingly, any of other common alfa-D-galactosidase inducers or substrates, e.g., D-galactose, melibiose and raffinose, did not stimulate its production. The crude alfa-D-galactosidase preparation was purified by anion-exchange chromatography and three isoenzymes with different substrate specificities were identified An extracellular alfa-D-galactosidase from Talaromyces flavus CCF 2686 with extremely broad and unusual acceptor specificity is produced exclusively in the presence of the specific inducer - 6-deoxy-D-glucose (quinovose). The procedure for the preparation of this very expensive substance has been modified and optimized. Surprisingly, any of other common alfa-D-galactosidase inducers or substrates, e.g., D-galactose, melibiose and raffinose, did not stimulate its production. The crude alfa-D-galactosidase preparation was purified by anion-exchange chromatography and three isoenzymes with different substrate specificities were identified (en) Extracelulární alfa-D-galaktosidasa z Talaromyces flavus CCF 2686 s extrémě širokou a neobvyklou substrátovou specifitou je produkována výhradně v přítomnosti specifického induktoru - 6-deoxy-D-glukosy (quinovosa). Enzym byl purifikován aniontově-výměnnou chromatografií a byly identifikovány 3 isoenzymy s rozdílnou substrátovou specifitou (cs)
Title	Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus Indukce a charakterizace neobvyklé Alfa-D-galaktosidasy z Talaromyces flavus (cs) Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus (en)
skos:prefLabel	Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus Indukce a charakterizace neobvyklé Alfa-D-galaktosidasy z Talaromyces flavus (cs) Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus (en)
skos:notation	RIV/61388971:_____/07:00088898!RIV08-AV0-61388971
http://linked.open.../vavai/riv/strany	61;71
http://linked.open...avai/riv/aktivita	N P Z
http://linked.open...avai/riv/aktivity	N, P(GA203/05/0172), P(LC06010), P(OC D25.001), P(OC D25.002), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Čechová, Ivana Simerská, Pavla Bezouška, Karel Křen, Vladimír Pelantová, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	426027
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/07:00088898
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	alfa-d-galactosidase; talaromyces flavus; enzyme purification (en)
http://linked.open.../riv/klicoveSlovo	alfa-d-galactosidase enzyme purification talaromyces flavus
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E3321360030F]
http://linked.open...i/riv/nazevZdroje	Journal of Biotechnology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Biocatalysis and Biotransformation http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20D25.002 a-N-Acetylgalactosaminidase and a-galactosidase-structural and functional characterisation, use in synthesis of biologically important glycoconjugates http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20D25.001
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	128
http://linked.open...iv/tvurceVysledku	Bezouška, Karel Křen, Vladimír Macková, M. Monti, D. Pelantová, Helena Riva, S. Čechová, Ivana Simerská, Pavla
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0168-1656
number of pages	11 (xsd:int)

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