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| rdf:type
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| Description
| - Mechanizmy, které řídí sebeobnovu a diferenciaci kmenových buněk, jsou komplexní a nejsou dosud zcela známé. Porozumění neurogenezi a diferenciaci nervových buněk přinese obrovský pokrok v léčbě onemocnění nervového systému. Pro lepší pochopení molekulárních mechanizmů diferenciace nervových kmenových buněk jsme v této práci využili kombinace výhodného modelu in vitro diferenciace fetálních prasečích nervových kmenových buněk (NSCs) a proteomické analýzy. Pomocí dvojrozměrné gelové elektroforézy následované hmotnostní spektrometrií jsme nejprve zmapovali proteiny konstitutivně přítomné v nervových kmenových buňkách a poté jsme identifikovali proteiny, jejichž hladina se významně zvyšuje či snižuje během diferenciace nervových buněk. Mezi konstitutivně přítomnými proteiny byly nejčastěji identifikovány proteiny účastnící se metabolismu a úprav RNA a proteinů, včetně chaperonů, následované proteiny hrající roli v buněčné organizaci (cytoskeletární proteiny a anexiny). Diferenciace nervových buněk byl... (cs)
- The mechanisms that regulate the maintenance of stem cell self-renewal versus differentiation are complex and remain mostly unknown. Understanding neurogenesis and neural cell differentiation presents a unique challenge for the treatment of nervous system disorders. To gain more insight into molecular mechanisms of the differentiation of neural cells, we combined the advantage of porcine fetal neural stem cells (NSCs) in vitro differentiation model and proteomic analysis. Using 2-DE followed by MS, we profiled constituent proteins of NSCs and their differentiated progenies at first and then indicated protein species that were significantly up- or downregulated during the differentiation. The largest identified group of constituent proteins was related to RNA and protein metabolism and processing, including chaperones, and the second largest consisted of proteins involved in cell organization (cytoskeleton and annexins). Differentiation of neural cells was found to be accompanied by changes in the ...
- The mechanisms that regulate the maintenance of stem cell self-renewal versus differentiation are complex and remain mostly unknown. Understanding neurogenesis and neural cell differentiation presents a unique challenge for the treatment of nervous system disorders. To gain more insight into molecular mechanisms of the differentiation of neural cells, we combined the advantage of porcine fetal neural stem cells (NSCs) in vitro differentiation model and proteomic analysis. Using 2-DE followed by MS, we profiled constituent proteins of NSCs and their differentiated progenies at first and then indicated protein species that were significantly up- or downregulated during the differentiation. The largest identified group of constituent proteins was related to RNA and protein metabolism and processing, including chaperones, and the second largest consisted of proteins involved in cell organization (cytoskeleton and annexins). Differentiation of neural cells was found to be accompanied by changes in the ... (en)
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| Title
| - A proteomic approach to studying the differentiation of neural stem cells
- A proteomic approach to studying the differentiation of neural stem cells (en)
- Proteomický přístup ke studiu diferenciace nervových kmenových buněk (cs)
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| skos:prefLabel
| - A proteomic approach to studying the differentiation of neural stem cells
- A proteomic approach to studying the differentiation of neural stem cells (en)
- Proteomický přístup ke studiu diferenciace nervových kmenových buněk (cs)
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| skos:notation
| - RIV/61388971:_____/07:00088692!RIV08-AV0-61388971
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(1M0538), P(LC545), Z(AV0Z40310501), Z(AV0Z50200510), Z(AV0Z50450515)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/07:00088692
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - differentiation; neural stem cells; two-dimensional gel electrophoresis (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Halada, Petr
- Motlík, Jan
- Vodička, Petr
- Kovářová, Hana
- Skalníková, Helena
- Řehulka, Pavel
- Chmelík, Josef
- Horning, O.
- Norregaard Jensen, O.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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