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rdf:type
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Description
| - Delecí genu aceE, kódujícího enzym E1p pyruvátdehydrogenasového komplexu, byl zkonstruován kmen Corynebacterium glutamicum aceE. V nerostoucí buněčné kultuře vykazoval kmen C. glutamicum aceE relativně vysokou vnitrobuněčnou koncentraci pyruvátu (25,9 mM). V kmeni C. glutamicum aceE byla zvýšena exprese genů ilvBNCE, kódujících enzymy syntasu acetohydroxykyselin, isomeroreduktasu a transaminasu B biosyntetické dráhy valinu, jejich klonovaním na plazmidu. Tento zásah vedl k přibližně 10-tinásobnému snížení vnitrobuněčné koncentrace pyruvátu (2.3 mM). Za podmínek fed-batch fermentace, vysoké hustoty buněčné kultury a v přítomnosti glukosy produkoval kmen C. glutamicum aceE(pJC4ilvBNCE) až 210 mM L-valin. (cs)
- A strain was Corynebacterium glutamicum aceE was constructed by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex. In the absence of cellular growth, C. glutamicum aceE showed a relatively high intracellular concentration of pyruvate (25.9 mM). Plasmid-bound overexpression of the ilvBNCE genes in C. glutamicum aceE, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase and transaminase B, resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM). In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum aceE(pJC4ilvBNCE) produced up to 210 mM L-valine
- A strain was Corynebacterium glutamicum aceE was constructed by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex. In the absence of cellular growth, C. glutamicum aceE showed a relatively high intracellular concentration of pyruvate (25.9 mM). Plasmid-bound overexpression of the ilvBNCE genes in C. glutamicum aceE, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase and transaminase B, resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM). In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum aceE(pJC4ilvBNCE) produced up to 210 mM L-valine (en)
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Title
| - L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum
- Produkce L-valinu bakterií Corynebacterium glutamicum deficientní na pyruvátdehydrogenasový komplex (cs)
- L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum (en)
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skos:prefLabel
| - L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum
- Produkce L-valinu bakterií Corynebacterium glutamicum deficientní na pyruvátdehydrogenasový komplex (cs)
- L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum (en)
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skos:notation
| - RIV/61388971:_____/07:00085754!RIV08-AV0-61388971
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/07:00085754
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - corynebacterium glutamicum; plasmid-bound overexpression; fed-batch fermentations (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Applied and Environmental Microbiology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Holátko, Jiří
- Eikmanns, B. J.
- Schreiner, M. E.
- Bartek, T.
- Blombach, B.
- Oldiges, M.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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