About: L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum     Goto   Sponge   NotDistinct   Permalink

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Description
  • Delecí genu aceE, kódujícího enzym E1p pyruvátdehydrogenasového komplexu, byl zkonstruován kmen Corynebacterium glutamicum aceE. V nerostoucí buněčné kultuře vykazoval kmen C. glutamicum aceE relativně vysokou vnitrobuněčnou koncentraci pyruvátu (25,9 mM). V kmeni C. glutamicum aceE byla zvýšena exprese genů ilvBNCE, kódujících enzymy syntasu acetohydroxykyselin, isomeroreduktasu a transaminasu B biosyntetické dráhy valinu, jejich klonovaním na plazmidu. Tento zásah vedl k přibližně 10-tinásobnému snížení vnitrobuněčné koncentrace pyruvátu (2.3 mM). Za podmínek fed-batch fermentace, vysoké hustoty buněčné kultury a v přítomnosti glukosy produkoval kmen C. glutamicum aceE(pJC4ilvBNCE) až 210 mM L-valin. (cs)
  • A strain was Corynebacterium glutamicum aceE was constructed by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex. In the absence of cellular growth, C. glutamicum aceE showed a relatively high intracellular concentration of pyruvate (25.9 mM). Plasmid-bound overexpression of the ilvBNCE genes in C. glutamicum aceE, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase and transaminase B, resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM). In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum aceE(pJC4ilvBNCE) produced up to 210 mM L-valine
  • A strain was Corynebacterium glutamicum aceE was constructed by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex. In the absence of cellular growth, C. glutamicum aceE showed a relatively high intracellular concentration of pyruvate (25.9 mM). Plasmid-bound overexpression of the ilvBNCE genes in C. glutamicum aceE, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase and transaminase B, resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM). In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum aceE(pJC4ilvBNCE) produced up to 210 mM L-valine (en)
Title
  • L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum
  • Produkce L-valinu bakterií Corynebacterium glutamicum deficientní na pyruvátdehydrogenasový komplex (cs)
  • L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum (en)
skos:prefLabel
  • L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum
  • Produkce L-valinu bakterií Corynebacterium glutamicum deficientní na pyruvátdehydrogenasový komplex (cs)
  • L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum (en)
skos:notation
  • RIV/61388971:_____/07:00085754!RIV08-AV0-61388971
http://linked.open.../vavai/riv/strany
  • 2079;2084
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • N, Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika
  • 7
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 431553
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/07:00085754
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • corynebacterium glutamicum; plasmid-bound overexpression; fed-batch fermentations (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [3F4070B6ED84]
http://linked.open...i/riv/nazevZdroje
  • Applied and Environmental Microbiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 73
http://linked.open...iv/tvurceVysledku
  • Holátko, Jiří
  • Eikmanns, B. J.
  • Schreiner, M. E.
  • Bartek, T.
  • Blombach, B.
  • Oldiges, M.
http://linked.open...n/vavai/riv/zamer
issn
  • 0099-2240
number of pages
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