About: Single-cell chlorophyll fluorescence kinetic microscopy of Pylaiella littoralis (Phaeophyceae) infected by Chytridium polysiphoniae (Chytridiomycota)     Goto   Sponge   NotDistinct   Permalink

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Description
  • Chlorophyll fluorescence imaging is a non-invasive method to monitor the metabolic state of photosynthetic organisms. We used spatially resolved (imaging) microscopic measurements of chlorophyll fluorescence kinetics to follow the fate of individual cells of the filamentous brown alga Pylaiella littoralis infected by the biotrophic parasite Chytridium polysiphoniae (Chytridiomycota). These measurements showed strong differences between individual parameters of the inhibition of photosynthesis, revealing important details about the mechanism of damage. The dark-adapted photochemistry of photosystem II itself (measured as Fv/Fm, where Fv0,25Fm_F0) remained unaffected until a very late stage of damage to the cell, while the light-adapted efficiency of PSII electron transport decreased earlier. A particularly complex pattern was found for the changes in nonphotochemical quenching (NPQ)
  • Chlorophyll fluorescence imaging is a non-invasive method to monitor the metabolic state of photosynthetic organisms. We used spatially resolved (imaging) microscopic measurements of chlorophyll fluorescence kinetics to follow the fate of individual cells of the filamentous brown alga Pylaiella littoralis infected by the biotrophic parasite Chytridium polysiphoniae (Chytridiomycota). These measurements showed strong differences between individual parameters of the inhibition of photosynthesis, revealing important details about the mechanism of damage. The dark-adapted photochemistry of photosystem II itself (measured as Fv/Fm, where Fv0,25Fm_F0) remained unaffected until a very late stage of damage to the cell, while the light-adapted efficiency of PSII electron transport decreased earlier. A particularly complex pattern was found for the changes in nonphotochemical quenching (NPQ) (en)
  • Mikroskopickým zobrazováním kinetiky fluorescence chlorofylu v buňkách vláknité hnědé řasy Pylaiella littoralis se studovaly změny ve fotosyntetickém aparátu při infekci parasitem Chytridium polysiphoniae. Výsledky ukazují, že infekce má vliv na regulaci fotosyntézy; v prvé fázi infekce fotosyntézu stimuluje, v druhé fázi všechny fotosyntetické parametry klesají. Fluorescenční parametry měřené na buňkách adaptovaných na tmu klesaly až v posledních stadiích poškození buňky (cs)
Title
  • Single-cell chlorophyll fluorescence kinetic microscopy of Pylaiella littoralis (Phaeophyceae) infected by Chytridium polysiphoniae (Chytridiomycota)
  • Mikroskopické sledování kinetiky fluorescence u jednotlivých buněk Pylaiella litoralis (Phaeophyceae) které infikovalo Chytridium polysiphoniae (Chytridiomycota) (cs)
  • Single-cell chlorophyll fluorescence kinetic microscopy of Pylaiella littoralis (Phaeophyceae) infected by Chytridium polysiphoniae (Chytridiomycota) (en)
skos:prefLabel
  • Single-cell chlorophyll fluorescence kinetic microscopy of Pylaiella littoralis (Phaeophyceae) infected by Chytridium polysiphoniae (Chytridiomycota)
  • Mikroskopické sledování kinetiky fluorescence u jednotlivých buněk Pylaiella litoralis (Phaeophyceae) které infikovalo Chytridium polysiphoniae (Chytridiomycota) (cs)
  • Single-cell chlorophyll fluorescence kinetic microscopy of Pylaiella littoralis (Phaeophyceae) infected by Chytridium polysiphoniae (Chytridiomycota) (en)
skos:notation
  • RIV/61388971:_____/06:00099737!RIV08-AV0-61388971
http://linked.open.../vavai/riv/strany
  • 395;403
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 499498
http://linked.open...ai/riv/idVysledku
  • RIV/61388971:_____/06:00099737
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • photosystem II; fluorescence kinetic microscopy; pylaiella littoralis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [D085FE2674CE]
http://linked.open...i/riv/nazevZdroje
  • European Journal of Phycology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 41
http://linked.open...iv/tvurceVysledku
  • Gachon, C. M. M.
  • Küpper, H.
  • Šetlík, Ivan
  • Kupper, F. C.
http://linked.open...n/vavai/riv/zamer
issn
  • 0967-0262
number of pages
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